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. 2022 Jan;151(1):125-142.
doi: 10.1007/s11120-021-00870-y. Epub 2021 Oct 20.

Complete genome of the thermophilic purple sulfur Bacterium Thermochromatium tepidum compared to Allochromatium vinosum and other Chromatiaceae

W Matthew Sattley  1 , Wesley D Swingley  2 , Brad M Burchell  3 , Emma D Dewey  3 , Mackenzie K Hayward  3 , Tara L Renbarger  3 , Kathryn N Shaffer  3 , Lynn M Stokes  3 , Sonja A Gurbani  2 , Catrina M Kujawa  2 , D Adam Nuccio  2 , Jacob Schladweiler  2 , Jeffrey W Touchman  4 , Zheng-Yu Wang-Otomo  5 , Robert E Blankenship  6 , Michael T Madigan  7
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Complete genome of the thermophilic purple sulfur Bacterium Thermochromatium tepidum compared to Allochromatium vinosum and other Chromatiaceae

W Matthew Sattley et al. Photosynth Res. 2022 Jan.

Abstract

The complete genome sequence of the thermophilic purple sulfur bacterium Thermochromatium tepidum strain MCT (DSM 3771T) is described and contrasted with that of its mesophilic relative Allochromatium vinosum strain D (DSM 180T) and other Chromatiaceae. The Tch. tepidum genome is a single circular chromosome of 2,958,290 base pairs with no plasmids and is substantially smaller than the genome of Alc. vinosum. The Tch. tepidum genome encodes two forms of RuBisCO and contains nifHDK and several other genes encoding a molybdenum nitrogenase but lacks a gene encoding a protein that assembles the Fe-S cluster required to form a functional nitrogenase molybdenum-iron cofactor, leaving the phototroph phenotypically Nif-. Tch. tepidum contains genes necessary for oxidizing sulfide to sulfate as photosynthetic electron donor but is genetically unequipped to either oxidize thiosulfate as an electron donor or carry out assimilative sulfate reduction, both of which are physiological hallmarks of Alc. vinosum. Also unlike Alc. vinosum, Tch. tepidum is obligately phototrophic and unable to grow chemotrophically in darkness by respiration. Several genes present in the Alc. vinosum genome that are absent from the genome of Tch. tepidum likely contribute to the major physiological differences observed between these related purple sulfur bacteria that inhabit distinct ecological niches.

Keywords: Anoxygenic phototrophic bacteria; Chromatiaceae; Hot spring; Purple sulfur bacteria; Thermochromatium tepidum; Thermophile.

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