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. 2021 Oct 20;12(1):31.
doi: 10.1186/s43008-021-00081-z.

The complete mitochondrial genome of Ophiocordyceps gracilis and its comparison with related species

Aifeire Abuduaini #  1 , Yuan-Bing Wang #  2 , Hui-Ying Zhou  1 , Rui-Ping Kang  1 , Ming-Liang Ding  3 , Yu Jiang  4 , Fei-Ya Suo  1 , Luo-Dong Huang  5   6
Affiliations

The complete mitochondrial genome of Ophiocordyceps gracilis and its comparison with related species

Aifeire Abuduaini et al. IMA Fungus. .

Abstract

In this study, the complete mitochondrial genome of O. gracilis was sequenced and assembled before being compared with related species. As the second largest mitogenome reported in the family Ophiocordycipitaceae, the mitogenome of O. gracilis (voucher OG201301) is a circular DNA molecule of 134,288 bp that contains numerous introns and longer intergenomic regions. UCA was detected as anticodon in tRNA-Sec of O. gracilis, while comparative mitogenome analysis of nine Ophiocordycipitaceae fungi indicated that the order and contents of PCGs and rRNA genes were considerably conserved and could descend from a common ancestor in Ophiocordycipitaceae. In addition, the expansion of mitochondrial organization, introns, gene length, and order of O. gracilis were determined to be similar to those of O. sinensis, which indicated common mechanisms underlying adaptive evolution in O. gracilis and O. sinensis. Based on the mitochondrial gene dataset (15 PCGs and 2 RNA genes), a close genetic relationship between O. gracilis and O. sinensis was revealed through phylogenetic analysis. This study is the first to investigate the molecular evolution, phylogenetic pattern, and genetic structure characteristics of mitogenome in O. gracilis. Based on the obtained results, the mitogenome of O. gracilis can increase understanding of the genetic diversity and evolution of cordycipitoid fungi.

Keywords: Mitochondrial genome; Ophiocordyceps gracilis; Ophiocordycipitaceae; Phylogenetic analysis.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Mitogenome map of Ophiocordyceps gracilis and O. sinensis. The outer layer represents O. sinensis obtained from Kang et al. (2017); the inner layer represents O. gracilis from this study. The genes are shown in different color blocks
Fig. 2
Fig. 2
Putative secondary structures of tRNAs from Ophiocordyceps gracilis mitogenomes. The amino acid accepter (AA) arm is shown in red; the TψC arm is shown in blue; the anticodon (AC) arm is shown in pink, and the dilhydoroudine (DHU) arm is shown in green
Fig. 3
Fig. 3
Collinearity analysis of nine mitogenomes from Ophiocordycipitaceae. Homologous regions between different mitogenomes are represented by the same color blocks linked by lines
Fig. 4
Fig. 4
Variation in the length and base composition of each of 15 protein-coding genes (PCGs) between Ophiocordyceps gracilis and O. sinensis (Kang et al. 2017). a PCGs length variation; b GC content; c AT-stew; d GC-stew. Light sea green represents O. gracilis, and orange represents O. sinensis
Fig. 5
Fig. 5
Relative synonymous codon usage (RSCU) in PCGs of Ophiocordyceps gracilis and O. sinensis (Kang et al. 2017)
Fig. 6
Fig. 6
Phylogenetic relationships of Ophiocordyceps gracilis and 23 other taxa of Hypocreales inferred from mitogenomes based on the Bayesian inference. Ophiocordyceps is framed with a dotted blue line. The species and GenBank accession number for mitogenomes used in the phylogenetic analysis are provided in Additional file 10: Table S9
See this image and copyright information in PMC

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