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. 2021 Oct 5;9(10):1398.
doi: 10.3390/biomedicines9101398.

T-Cadherin and the Ratio of Its Ligands as Predictors of Carotid Atherosclerosis: A Pilot Study

Alexander Balatskiy  1   2 , Marina Teterina  3 , Alexandra Pisaryuk  3 , Irina Balabanenko  1 , Alexey Kadrev  1   4 , Anastasia Tishuk  1 , Maria Balatskaya  5 , Larisa Samokhodskaya  1 , Sergey Boytsov  6 , Natalia Kalinina  5 , Vsevolod Tkachuk  1   5   6
Affiliations

T-Cadherin and the Ratio of Its Ligands as Predictors of Carotid Atherosclerosis: A Pilot Study

Alexander Balatskiy et al. Biomedicines. .

Abstract

In the cardiovascular system, atherogenic low-density lipoproteins (LDL) and the protective hormone adiponectin bind to the same receptor, T-cadherin. In this study, we tested the hypothesis that the ratio of circulating LDL to high-molecular weight (HMW) adiponectin could predict the development of atherosclerosis. Using enzyme-linked immunosorbent assay, we measured the level of circulating HMW adiponectin in the blood of donors together with ultrasound measuring of intima-media thickness (IMT) of carotid arteries. Single-nucleotide polymorphisms in the T-cadherin gene were identified using polymerase chain reaction. We found that carotid artery IMT is inversely correlated with the level of HMW in male subjects. We also found that the G allele of rs12444338 SNP in the T-cadherin gene correlates with a lower level of circulating T-cadherin and thinner IMT and therefore could be considered as an atheroprotective genotype. Despite our data, we could not provide direct evidence for the initial study hypothesis. However, we did uncover an important correlation between circulating T-cadherin and thinner carotid IMT.

Keywords: T-cadherin; atherosclerosis; high molecular weight adiponectin; intima-media thickness.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Association between presence of thick (>0.9 mm) carotid intima-media complex and (a) plasma LDL level (p = 0.661), (b) plasma ApoB level (p = 0.711), (c) plasma HMW adiponectin level (p = 0.469), (d) plasma T-cadherin level (0.853), (e) HMW adiponectin/LDL molar ratio (0.578), and (f) HMW adiponectin/ApoB molar ratio (p = 0.625). n = 35 for all diagrams.
Figure 2
Figure 2
Association between presence of AH and (a) plasma LDL level (p = 0.609), (b) plasma ApoB level (p = 0.668), (c) plasma HMW adiponectin level (p = 0.779), (d) plasma T-cadherin level (0.181), (e) HMW adiponectin/LDL molar ratio (0.961), and (f) HMW adiponectin/ApoB molar ratio (p = 0.779). n = 35 for all diagrams.
Figure 3
Figure 3
Significant differences between rs12051272 T allele carriers and non-carriers: subjects with G/T genotype have higher BMI (a) and lower HMW adiponectin/ApoB molar ratio (b). n = 35 for both diagrams.
Figure 4
Figure 4
Significant differences in IMT between rs12444338 G allele carriers and non-carriers: (a) obese patients (n = 10, p = 0.020) and (b) females (n = 25, p = 0.047).
Figure 5
Figure 5
CDH13 rs12444338 genotypes and T-cadherin plasma levels (n = 35).
See this image and copyright information in PMC

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