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Review
. 2022 Jan;49(1):657-673.
doi: 10.1007/s11033-021-06832-w. Epub 2021 Oct 23.

Insights of CRISPR-Cas systems in stem cells: progress in regenerative medicine

Shanmugam Dilip Kumar  1 , Manimaran Aashabharathi  2 , Guruviah KarthigaDevi  1 , Ramasamy Subbaiya  3 , Muthupandian Saravanan  4
Affiliations
Review

Insights of CRISPR-Cas systems in stem cells: progress in regenerative medicine

Shanmugam Dilip Kumar et al. Mol Biol Rep. 2022 Jan.

Abstract

Regenerative medicine, a therapeutic approach using stem cells, aims to rejuvenate and restore the normalized function of the cells, tissues, and organs that are injured, malfunctioning, and afflicted. This influential technology reaches its zenith when it is integrated with the CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) technology of genome editing. This tool acts as a programmable restriction enzyme system, which targets DNA as well as RNA and gets redeployed for the customization of DNA/RNA sequences. The dynamic behaviour of nuclear manipulation and transcriptional regulation by CRISPR-Cas technology renders it with numerous employment in the field of biologics and research. Here, the possible impact of the commonly practiced CRISPR-Cas systems in regenerative medicines is being reviewed. Primarily, the discussion of the working mechanism of this system and the fate of stem cells will be scrutinized. A detailed description of the CRISPR based regenerative therapeutic approaches for a horde of diseases like genetic disorders, neural diseases, and blood-related diseases is elucidated.

Keywords: CRISPR-associated proteins; Gene therapy; Genetic disorders; Genome editing; Protospacer adjacent motif; Stem cells.

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References

8. References

    1. Badylak SF, Nerem RM (2010) Progress in tissue engineering and regenerative medicine. Proc Natl Acad Sci USA 107(8):3285–3286. https://doi.org/10.1073/pnas.1000256107 - DOI - PubMed - PMC
    1. Wobma H, Vunjak-Novakovic G (2016) Tissue engineering and regenerative medicine 2015: a year in review. Tissue Eng B: Rev 22(2):101–113. https://doi.org/10.1089/ten.TEB.2015.0535 - DOI
    1. Park KM, Shin YM, Kim K, Shin H (2018) Tissue engineering and regenerative medicine 2017: a year in review. Tissue Eng B: Rev 24(5):327–344. https://doi.org/10.1089/ten.teb.2017.0081 - DOI
    1. Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K (2013) Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs. Cell Stem Cell 12(4):393. https://doi.org/10.1016/j.stem.2013.03.006 - DOI - PubMed - PMC
    1. Hendriks WT, Jiang X, Daheron L, Cowan CA (2015) TALEN-and CRISPR/Cas9-mediated gene editing in human pluripotent stem cells using lipid-based transfection. Curr Protoc Stem Cell Biol. https://doi.org/10.1016/j.stem.2015.12.002 - DOI - PubMed

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