Corrigendum: TRPC1 Inhibits Cell Proliferation/Invasion and Is Predictive of a Better Prognosis of Esophageal Squamous Cell Carcinoma

Abstract

[This corrects the article DOI: 10.3389/fonc.2021.627713.].

Keywords: TRPC1; cell proliferation; esophageal squamous cell carcinoma; migration and invasion; prognosis.

Figure 3

Knockdown of TRPC1 by transfection with siRNA promoted the proliferation, wound healing, migration, and invasion abilities of EC9706 cells. (A) Western blotting showed TRPC1 expression in four esophageal squamous carcinoma cell lines: Eca109, EC9706, KYSE510, and KYSE150. (B) Representative western blot showing the effect of siRNA directed against TRPC1 on the level of TRPC1 protein in EC9706. (C) Analysis of proliferation of cells transfected with siTRPC1 by CCK-8 assay. Cell proliferation was measured 24, 48, 72, and 96h post-transfection. After treatment with siTRPC1, the viability of EC9706 cells was significantly increased. (D) Cellular wound healing after knockdown of TRPC1 in EC9706 cells (magnification: 100×). The rate of wound healing of EC9706-siTRPC1-1 or EC9706-siTRPC1-2 cells was significantly higher than that of EC9706-siNC cells (P<0.01). (E) Cell migration and invasion after knockdown of TRPC1 (magnification: 100×). The cells of the silenced expression group (EC9706-siTRPC1-1 or EC9706-siTRPC1-2) had higher migration and invasion abilities than those of the control group (EC9706-siNC), (P<0.05). NC represented as negative control. *P < 0.05, **P < 0.01, ****P < 0.0001.