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. 2021 Sep 20;11(18):e4154.
doi: 10.21769/BioProtoc.4154.

Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing

Yang Yang  1 , Weixing Shan  1
Affiliations

Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing

Yang Yang et al. Bio Protoc. .

Abstract

Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and chloroplasts, such as poisoned primer extension (PPE) assays, high-resolution melting (HRM) analysis, and DNA sequencing. Here, we describe a method for the quantitative detection of RNA editing at specific sites by sequencing cDNA from plant leaves to further evaluate the effect of different treatments or plant mutants on the C to U RNA editing in mitochondria and chloroplasts.

Keywords: C to U RNA editing; Chloroplasts; DNA sequencing; Editing extent; Mitochondria.

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Conflict of interest statement

Competing interestsThe authors declare no conflict of interests.

Figures

Figure 1.
Figure 1.. Workflow of the RNA editing analysis of specific sites in mitochondria and chloroplasts
Figure 2.
Figure 2.. Analysis of RNA editing extent by Bioedit.
The blue arrows mark the targeted nucleotide peaks, and the black hollow boxes mark the reads of the peak heights.
See this image and copyright information in PMC

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