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. 2021 Dec;29(3-4):391-416.
doi: 10.1007/s10577-021-09674-1. Epub 2021 Oct 25.

Against the mainstream: exceptional evolutionary stability of ZW sex chromosomes across the fish families Triportheidae and Gasteropelecidae (Teleostei: Characiformes)

Cassia Fernanda Yano  1 , Alexandr Sember  2 , Rafael Kretschmer  1 , Luiz Antônio Carlos Bertollo  1 , Tariq Ezaz  3 , Terumi Hatanaka  1 , Thomas Liehr  4 , Petr Ráb  5 , Ahmed Al-Rikabi  4 , Patrik Ferreira Viana  6 , Eliana Feldberg  6 , Ezequiel Aguiar de Oliveira  1 , Gustavo Akira Toma  1 , Marcelo de Bello Cioffi  1
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Against the mainstream: exceptional evolutionary stability of ZW sex chromosomes across the fish families Triportheidae and Gasteropelecidae (Teleostei: Characiformes)

Cassia Fernanda Yano et al. Chromosome Res. 2021 Dec.

Abstract

Teleost fishes exhibit a breath-taking diversity of sex determination and differentiation mechanisms. They encompass at least nine sex chromosome systems with often low degree of differentiation, high rate of inter- and intra-specific variability, and frequent turnovers. Nevertheless, several mainly female heterogametic systems at an advanced stage of genetic differentiation and high evolutionary stability have been also found across teleosts, especially among Neotropical characiforms. In this study, we aim to characterize the ZZ/ZW sex chromosome system in representatives of the Triportheidae family (Triportheus auritus, Agoniates halecinus, and the basal-most species Lignobrycon myersi) and its sister clade Gasteropelecidae (Carnegiella strigata, Gasteropelecus levis, and Thoracocharax stellatus). We applied both conventional and molecular cytogenetic approaches including chromosomal mapping of 5S and 18S ribosomal DNA clusters, cross-species chromosome painting (Zoo-FISH) with sex chromosome-derived probes and comparative genomic hybridization (CGH). We identified the ZW sex chromosome system for the first time in A. halecinus and G. levis and also in C. strigata formerly reported to lack sex chromosomes. We also brought evidence for possible mechanisms underlying the sex chromosome differentiation, including inversions, repetitive DNA accumulation, and exchange of genetic material. Our Zoo-FISH experiments further strongly indicated that the ZW sex chromosomes of Triportheidae and Gasteropelecidae are homeologous, suggesting their origin before the split of these lineages (approx. 40-70 million years ago). Such extent of sex chromosome stability is almost exceptional in teleosts, and hence, these lineages afford a special opportunity to scrutinize unique evolutionary forces and pressures shaping sex chromosome evolution in fishes and vertebrates in general.

Keywords: CGH; Zoo-FISH; chromosome rearrangements; rDNA; sex chromosome evolution.

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