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. 2022 Apr;35(4):624-630.
doi: 10.5713/ab.21.0220. Epub 2021 Oct 21.

Expression levels of filaggrin-2 in relation to drip loss in pigs

Autchara Kayan  1 , Nunyarat Koomkrong  2
Affiliations

Expression levels of filaggrin-2 in relation to drip loss in pigs

Autchara Kayan et al. Anim Biosci. 2022 Apr.

Abstract

Objective: The aim of this study was to investigate the expression level of filaggrin-2 (FLG2) in correlation with drip loss.

Methods: The muscle samples were randomly taken from a local meat supplier. Samples were taken from Longissimus lumborum muscles to evaluate the drip loss (n = 100). Five muscles per group (low and high drip loss) were selected to evaluate FLG2 mRNA and protein expression levels.

Results: mRNA of FLG2 gene was not significantly different in pigs with different levels of drip loss (p>0.05). Statistical analysis revealed that FLG2 protein expression levels were significantly different between the drip loss groups. Western blot revealed that the high drip loss group had higher FLG2 protein expression level than the low drip loss group (p<0.001). Moreover, immunohistochemistry revealed the high signal intensity was on the muscle cell membrane and cytoplasm.

Conclusion: FLG2 protein might play roles in drip loss of pork and will provide the basis for information to improving meat quality traits in pigs.

Keywords: Drip Loss; Expression; Filaggrin-2 (FLG2); Pig.

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Figures

Figure 1
Figure 1
Expression level of FLG2 gene transcript in Longissimus lumborum muscle between low and high drip loss groups: RT-qPCR analysis of FLG2 gene expression in the Longissimus lumborum muscle from low drip loss (≤1.47%) and high drip loss (≥6.25%) groups (n = 5 per group) in pigs. TBP was used as a reference for normalization. The bars represent the means±standard error of the means. The mRNA expression of FLG2 gene was no significant difference between groups (p>0.05). FLG2, filaggrin-2; RT-qPCR, real-time quantitative polymerase chain reaction; TBP, TATA sequence binding protein.
Figure 2
Figure 2
FLG2 protein expression in low and high drip loss groups: Western blot analysis of FLG2 and GAPDH protein expression in the Longissimus lumborum muscle from low drip loss (≤1.47%) and high drip loss (≥6.25%) groups (n = 5 per group) in pigs. GAPDH was used as a reference for normalization. Relative band intensities were compared by determining the ratio of the area densities of FLG2 to GAPDH bands for each lane. The high drip loss group had higher FLG2 protein expression levels than the low drip loss group. FLG2, filaggrin-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Figure 3
Figure 3
FLG2 protein expression in muscle fiber of low and high drip loss groups: The immunohistochemical detection of FLG2 protein in formalin-fixed paraffin-embedded samples from the Longissimus lumborum muscle from low drip loss (≤1.47%) and high drip loss (≥6.25%) groups in pigs. Stained cross-sections were imaged with a light microscope at 100×. The higher signal intensity was observed on the muscle cell membrane and periphery of the cytoplasm in high drip loss group. FLG2, filaggrin-2.
See this image and copyright information in PMC

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