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. 2021 Nov-Dec;35(6):3449-3457.
doi: 10.21873/invivo.12645.

Embryonic Cell-free DNA in Spent Culture Medium: A Non-invasive Tool for Aneuploidy Screening of the Corresponding Embryos

Afrodite Sialakouma  1   2 , Ioannis Karakasiliotis  3 , Vaia Ntala  2 , Nikolaos Nikolettos  4 , Byron Asimakopoulos  2
Affiliations

Embryonic Cell-free DNA in Spent Culture Medium: A Non-invasive Tool for Aneuploidy Screening of the Corresponding Embryos

Afrodite Sialakouma et al. In Vivo. 2021 Nov-Dec.

Abstract

Background/aim: Preimplantation genetic testing (PGT) for chromosomal screening, based on embryo biopsy, has significant limitations. Cell-free DNA (cf-DNA) has been detected in spent culture medium (SCM), opening new horizons for the development of non-invasive PGT (ni-PGT). In this study, we evaluated the diagnostic performance of ni-PGT for aneuploidy (niPGT-A), comparing the results of trophectoderm biopsies (TE) and respective SCM from individually cultured embryos via Next Generation Sequencing (NGS).

Materials and methods: Forty fresh embryos were analyzed. TE and SCM from blastocysts were collected and analyzed.

Results: We detected cfDNA in 100% of samples tested. The overall concordance rate between the ni-PGT-A and PGT-A was 27/33 (81.8%). The full concordance rate was 21/33 (63.6%). The aneuploidy agreement was 91.66%, and the euploidy agreement was 76.19%.

Conclusion: We found a good accordance between TE and SCM analysis, suggesting that niPGT-A could be a reliable alternative for chromosomal abnormalities assessment of in vitro cultured embryos.

Keywords: cfDNA; culture medium; non-invasive PGT; trophectoderm biopsy.

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Conflict of interest statement

There are no conflicts of interest.

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Figure 1. The study flow chart.
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References

    1. Goldman MB, Thornton KL, Ryley D, Alper MM, Fung JL, Hornstein MD, Reindollar RH. A randomized clinical trial to determine optimal infertility treatment in older couples: the Forty and Over Treatment Trial (FORT-T) Fertil Steril. 2014;101(6):1574–81.e1-2. doi: 10.1016/j.fertnstert.2014.03.012. - DOI - PMC - PubMed
    1. Reindollar RH, Regan MM, Neumann PJ, Levine BS, Thornton KL, Alper MM, Goldman MB. A randomized clinical trial to evaluate optimal treatment for unexplained infertility: the fast track and standard treatment (FASTT) trial. Fertil Steril. 2010;94(3):888–899. doi: 10.1016/j.fertnstert.2009.04.022. - DOI - PubMed
    1. Fishel SB, Edwards RG, Evans CJ. Human chorionic gonadotropin secreted by preimplantation embryos cultured in vitro. Science. 1984;223(4638):816–818. doi: 10.1126/science.6546453. - DOI - PubMed
    1. Fishel SB, Edwards RG, Walters DE. Follicular steroids as a prognosticator of successful fertilization of human oocytes in vitro. J Endocrinol. 1983;99(2):335–344. doi: 10.1677/joe.0.0990335. - DOI - PubMed
    1. Fragouli E, Wells D. Aneuploidy in the human blastocyst. Cytogenet Genome Res. 2011;133(2-4):149–159. doi: 10.1159/000323500. - DOI - PubMed

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