Protein Phosphatase PP2C Identification in Entamoeba spp
- PMID: 34697588
- PMCID: PMC8541852
- DOI: 10.1155/2021/5746629
Protein Phosphatase PP2C Identification in Entamoeba spp
Abstract
Entamoeba histolytica is the causative agent of amoebiasis, and Entamoeba dispar is its noninvasive morphological twin. Entamoeba invadens is a reptilian parasite. In the present study, Western blot, phosphatase activity, immunofluorescence, and bioinformatic analyses were used to identify PP2C phosphatases of E. histolytica, E. dispar, and E. invadens. PP2C was identified in trophozoites of all Entamoeba species and cysts of E. invadens. Immunoblotting using a Leishmania mexicana anti-PP2C antibody recognized a 45.2 kDa PP2C in all species. In E. histolytica and E. invadens, a high molecular weight element PP2C at 75 kDa was recognized, mainly in cysts of E. invadens. Immunofluorescence demonstrated the presence of PP2C in membrane and vesicular structures in the cytosol of all species analyzed. The ~75 kDa PP2C of Entamoeba spp. shows the conserved domain characteristic of phosphatase enzymes (according to in silico analysis). Possible PP2C participation in the encystation process was discussed.
Copyright © 2021 Abril Navarrete-Mena et al.
Conflict of interest statement
The authors declare that they have no conflict of interest.
Figures





References
-
- World Health Organization. Amoebiasis. Weekly Epidemiological Record . 1997;72(14):97–99. - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous