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. 2022 Jan;35(1):e2942.
doi: 10.1002/jmr.2942. Epub 2021 Oct 25.

Investigation of polyacrylamide hydrogel-based molecularly imprinted polymers using protein gel electrophoresis

Hazim F El Sharif  1 , Flavio Giosia  1   2 , Subrayal M Reddy  1
Affiliations

Investigation of polyacrylamide hydrogel-based molecularly imprinted polymers using protein gel electrophoresis

Hazim F El Sharif et al. J Mol Recognit. 2022 Jan.

Abstract

In conjunction with polyacrylamide gel electrophoresis (PAGE), molecular imprinting methods have been applied to produce a multilayer mini-slab in order to evaluate how selectively and specifically a hydrogel-based molecularly imprinted polymer (MIP) binds bovine haemoglobin (BHb, ~64.5 kDa). A three-layer mini-slab comprising an upper and lower layer and a MIP, or a non-imprinted control polymer dispersion middle layer has been investigated. The discriminating MIP layer, also based on polyacrylamide, was able to specifically bind BHb molecules in preference to a protein similar in molecular weight such as bovine serum albumin (BSA, ~66 kDa). Protein staining allowed us to visualise the protein retention strength of the MIP layer under the influence of an electric field. This method could be applied to other proteins with implications in effective protein capture, disease diagnostics, and protein analysis.

Keywords: bovine haemoglobin; bovine serum albumin; hydrogel; molecularly imprinted polymers; multilayer gel; polyacrylamide gel electrophoresis; protein imprinting.

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References

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