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Comparative Study
. 1987 Feb 13;142(3):1095-103.
doi: 10.1016/0006-291x(87)91528-2.

Purification and characterization of erythroid differentiation factor (EDF) isolated from human leukemia cell line THP-1

Y Eto, T Tsuji, M Takezawa, S Takano, Y Yokogawa, H Shibai
Comparative Study

Purification and characterization of erythroid differentiation factor (EDF) isolated from human leukemia cell line THP-1

Y Eto et al. Biochem Biophys Res Commun. .

Erratum in

  • Biochem Biophys Res Commun 1987 May 14;144(3):1349

Abstract

We isolated a protein, from a cell line of human origin, which exhibits extensive differentiation inducing activity toward Friend leukemia cells. The protein, called Erythroid Differentiation Factor (EDF), was found in a 4 day culture of THP-1 cells performed in the presence of 4 beta-phorbol 12-myristate 13-acetate(PMA). EDF is a homodimer of a molecular weight of 25,000, with an NH2-terminal sequence identical to that of the beta A-chain of porcine Inhibin. It was suggested that a single protein species is responsible for the activities of both EDF and FRP, a FSH releasing protein isolated from porcine ovarian follicular fluid.

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