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Review
. 2022:2382:1-16.
doi: 10.1007/978-1-0716-1744-1_1.

Methods to Visualize the Actin Cytoskeleton During Plant Cell Division

Marie-Cécile Caillaud  1
Affiliations
Review

Methods to Visualize the Actin Cytoskeleton During Plant Cell Division

Marie-Cécile Caillaud. Methods Mol Biol. 2022.

Abstract

Cell division in plants consists of separating the mother cell in two daughter cells by the centrifugal growth of a new wall. This process involves the reorganization of the structural elements of the cell, namely the microtubules and actin cytoskeleton which allow the coordination, the orientation, and the progression of mitosis. In addition to its implication in those plant-specific structures, the actin cytoskeleton, in close association with the plasma membrane, exhibits specific patterning at the cortex of the dividing cells, and might act as a signaling component. This review proposes an overview of the techniques available to visualize the actin cytoskeleton in fixed tissues or living cells during division, including electron, fluorescent, and super-resolution microscopy techniques.

Keywords: Actin; Cell biology; Electron tomography; Fluorescent-tagged reporters; Immunogold labeling; Phallotoxins; Plant; Super-resolution microscopy.

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