Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Peng Wang^{1

2

3}, Shouxin Liu^{4

5

6}, Xiaochun Wang⁷, Qinqin Cong⁷, Jialin Lu⁷

Affiliations

¹ College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, 050018, China. pwang@hebust.edu.cn.
² State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science & Technology, Shijiazhuang, 050018, China. pwang@hebust.edu.cn.
³ Hebei Province Pharmaceutical Chemical Engineering Technology Research Center, Shijiazhuang, 050018, China. pwang@hebust.edu.cn.
⁴ College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, 050018, China. chlsx@263.net.
⁵ State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science & Technology, Shijiazhuang, 050018, China. chlsx@263.net.
⁶ Hebei Province Pharmaceutical Chemical Engineering Technology Research Center, Shijiazhuang, 050018, China. chlsx@263.net.
⁷ College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, 050018, China.

PMID: 34706751
PMCID: PMC8554860
DOI: 10.1186/s13036-021-00275-4

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Peng Wang et al. J Biol Eng. 2021.

. 2021 Oct 27;15(1):25.

doi: 10.1186/s13036-021-00275-4.

Authors

Peng Wang^{1

2

3}, Shouxin Liu^{4

5

6}, Xiaochun Wang⁷, Qinqin Cong⁷, Jialin Lu⁷

Affiliations

¹ College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, 050018, China. pwang@hebust.edu.cn.
² State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science & Technology, Shijiazhuang, 050018, China. pwang@hebust.edu.cn.
³ Hebei Province Pharmaceutical Chemical Engineering Technology Research Center, Shijiazhuang, 050018, China. pwang@hebust.edu.cn.
⁴ College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, 050018, China. chlsx@263.net.
⁵ State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science & Technology, Shijiazhuang, 050018, China. chlsx@263.net.
⁶ Hebei Province Pharmaceutical Chemical Engineering Technology Research Center, Shijiazhuang, 050018, China. chlsx@263.net.
⁷ College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, 050018, China.

PMID: 34706751
PMCID: PMC8554860
DOI: 10.1186/s13036-021-00275-4

Abstract

Background: The Paracoccus sp. strain isolated from sludge was identified and evaluated for catalytic activity in the degradation of penicillin G.

Results: High degradation efficiency and synergistic catalytic effects of the whole cell and visible light without additional catalysts were observed. The key factors influencing the degradation and kinetics of penicillin G were investigated. The results showed the phenylacetic acid, which was produced during penicillin G biodegradation, exhibited stronger inhibiting effects on KDSPL-02. However, this effect was reduced by visible light irradiation without any additional photocatalyst; furthermore, the rate of penicillin G biodegradation was accelerated, reaching a 100% rate in 12 h at a penicillin G concentration of 1.2 g/L. Four key intermediates produced during penicillin G degradation were isolated and identified by LC-MS, ¹H NMR, and ¹³C NMR. Enzymes involved in the PAA pathway were proposed from a genomic analysis of KDSPL-02.

Conclusions: These results provide a new method for bio-degrading of penicillin or other antibiotic pollutants using photoaccelerating biocatalysts with greater efficiency and more environmentally friendly conditions.

Keywords: Biodegradation; Paracoccus sp. strain; Penicillin G; Synergistic catalysis; Visible light.

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Conflict of interest statement

Not applicable.

Figures

**Fig. 1**
the growth of KDSPL-02 when using varies substance (glucose, sucrose, starch or penicillin G) as the sole carbon and energy source

**Fig. 2**
Effect of cell dry weight of KDSPL-02 whole cell (a), initial penicillin G concentration (b), temperature (c), and pH (d) on degradation rate by *Paracoccus sp*. KDSPL-02

**Fig. 3**
Conversion of penicillin G biodegradation at initial penicillin G concentrations of 0.8 g L^− 1 (a) and 1.6 g L^− 1 (b)

**Fig. 4**
Proposed penicillin G biodegradation pathway by *Paracoccus sp.* KDSPL-02

**Fig. 5**
Conversion of penicillin G biodegradation at an initial penicillin G concentration of 1.2 g L^− 1 (a) and using co-catalysis of KDSPL-02 whole cell and light degradation at an initial penicillin G concentration of 1.2 g L^− 1 (b)

**Fig. 6**
Effect of visible light inducing catalysis on phenylacetic acid oxidation

See this image and copyright information in PMC

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Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Affiliations

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

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References

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