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. 2021 Oct 27;15(1):25.
doi: 10.1186/s13036-021-00275-4.

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Peng Wang  1   2   3 , Shouxin Liu  4   5   6 , Xiaochun Wang  7 , Qinqin Cong  7 , Jialin Lu  7
Affiliations

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Peng Wang et al. J Biol Eng. .

Abstract

Background: The Paracoccus sp. strain isolated from sludge was identified and evaluated for catalytic activity in the degradation of penicillin G.

Results: High degradation efficiency and synergistic catalytic effects of the whole cell and visible light without additional catalysts were observed. The key factors influencing the degradation and kinetics of penicillin G were investigated. The results showed the phenylacetic acid, which was produced during penicillin G biodegradation, exhibited stronger inhibiting effects on KDSPL-02. However, this effect was reduced by visible light irradiation without any additional photocatalyst; furthermore, the rate of penicillin G biodegradation was accelerated, reaching a 100% rate in 12 h at a penicillin G concentration of 1.2 g/L. Four key intermediates produced during penicillin G degradation were isolated and identified by LC-MS, 1H NMR, and 13C NMR. Enzymes involved in the PAA pathway were proposed from a genomic analysis of KDSPL-02.

Conclusions: These results provide a new method for bio-degrading of penicillin or other antibiotic pollutants using photoaccelerating biocatalysts with greater efficiency and more environmentally friendly conditions.

Keywords: Biodegradation; Paracoccus sp. strain; Penicillin G; Synergistic catalysis; Visible light.

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Conflict of interest statement

Not applicable.

Figures

Fig. 1
Fig. 1
the growth of KDSPL-02 when using varies substance (glucose, sucrose, starch or penicillin G) as the sole carbon and energy source
Fig. 2
Fig. 2
Effect of cell dry weight of KDSPL-02 whole cell (a), initial penicillin G concentration (b), temperature (c), and pH (d) on degradation rate by Paracoccus sp. KDSPL-02
Fig. 3
Fig. 3
Conversion of penicillin G biodegradation at initial penicillin G concentrations of 0.8 g L− 1 (a) and 1.6 g L− 1 (b)
Fig. 4
Fig. 4
Proposed penicillin G biodegradation pathway by Paracoccus sp. KDSPL-02
Fig. 5
Fig. 5
Conversion of penicillin G biodegradation at an initial penicillin G concentration of 1.2 g L− 1 (a) and using co-catalysis of KDSPL-02 whole cell and light degradation at an initial penicillin G concentration of 1.2 g L− 1 (b)
Fig. 6
Fig. 6
Effect of visible light inducing catalysis on phenylacetic acid oxidation
See this image and copyright information in PMC

References

    1. von Dach E, Albrich WC, Brunel AS, Prendki V, Cuvelier C, Flury D, et al. Effect of C-reactive protein-guided antibiotic treatment duration, 7-day treatment, or 14-day treatment on 30-day clinical failure rate in patients with uncomplicated gram-negative bacteremia: a randomized clinical trial. JAMA. 2020;323(21):2160–2169. doi: 10.1001/jama.2020.6348. - DOI - PMC - PubMed
    1. Tian Y, Li G, Zhang H, Xu L, Jiao A, Chen F, et al. Construction of optimized au@ag core-shell nanorods for ultralow SERS detection of antibiotic levofloxacin molecules. Opt Express. 2018;26(18):23347–23358. doi: 10.1364/OE.26.023347. - DOI - PubMed
    1. Zhang Y, Liu H, Dai X, Cai C, Wang J, Wang M, et al. Impact of application of heat-activated persulfate oxidation treated erythromycin fermentation residue as a soil amendment: soil chemical properties and antibiotic resistance. Sci Total Environ. 2020;736:139668. doi: 10.1016/j.scitotenv.2020.139668. - DOI - PubMed
    1. Jalili R, Khataee A, Rashidi MR, Razmjou A. Detection of penicillin G residues in milk based on dual-emission carbon dots and molecularly imprinted polymers. Food Chem. 2020;314:126172. doi: 10.1016/j.foodchem.2020.126172. - DOI - PubMed
    1. Balaban NQ, Helaine S, Lewis K, Ackermann M, Aldridge B, Andersson DI, et al. Definitions and guidelines for research on antibiotic persistence. Nat Rev Microbiol. 2019;17(7):441–448. doi: 10.1038/s41579-019-0196-3. - DOI - PMC - PubMed

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