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. 2022:2409:11-30.
doi: 10.1007/978-1-0716-1879-0_2.

Transmission Electron Microscopy Studies of Dengue Viruses

Gabriela Cardoso Caldas  1 , Ortrud Monika Barth  2 , Debora Ferreira Barreto-Vieira  2
Affiliations

Transmission Electron Microscopy Studies of Dengue Viruses

Gabriela Cardoso Caldas et al. Methods Mol Biol. 2022.

Abstract

Despite the advancement of molecular biology techniques, morphological studies using transmission electron microscopy (TEM) are still being of great importance to elucidate some aspects of viral structures, morphogenesis, and pathogenesis. In relation to dengue viruses (DENV), several studies report the use of TEM to obtain a clearer definition of viral morphology, the events involved in its morphogenesis, aspects of pathogenesis, and cell tropism. In this chapter, the main technical protocols and their respective reagents for studies of DENV infection by TEM are described, both in cell culture and in biological tissue samples.

Keywords: Fixation of biological samples; Positive and negative staining technique; Transmission electron microscopy; Ultramicrotomy; Ultrastructural analysis.

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