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. 2021 Oct 11:8:1783-1791.
doi: 10.1016/j.toxrep.2021.10.010. eCollection 2021.

Genotoxicity and 28-day repeated dose oral toxicity study of ovatodiolide in rats

Jing-Chun Chen  1 , Yan-Zhen Dai  2 , Yew-Min Tzeng  3 , Jiunn-Wang Liao  1   4
Affiliations

Genotoxicity and 28-day repeated dose oral toxicity study of ovatodiolide in rats

Jing-Chun Chen et al. Toxicol Rep. .

Abstract

Ovatodiolide is a bioactive cembrane-type diterpenoid isolated from Anisomeles indica (L.) Kuntze. It has been proven that ovatodiolide is anti-inflammatory, anti-tumorigenic, anti-melanogenic and attenuates asthma by regulating signaling pathways. The aim of this study was to evaluate the safety of ovatodiolide by conducting genotoxicity tests and 28-day oral toxicity tests in rats. Genotoxicity assays were conducted by using a bacterial reverse mutation test and mammalian chromosomal aberration test to assess whether ovatodiolide causes reverse mutations and mutagenicity with or without metabolism activation. For the in vivo mammalian erythrocyte micronucleus test, mice were administered a single dose of 0, 250, 500 or 1000 mg/kg b.w. ovatodiolide by single gavage. In the acute oral toxicity test, rats were given a single dose of ovatodiolide 1000 mg/kg b.w. by single gavage. In the 28-day oral toxicity test, groups were divided into a control, ovatodiolide 10, 25 and 50 mg/kg b.w. The results showed that there was no mutagenicity in the bacterial reverse mutation test or the mammalian chromosomal aberration test with or without S9 fraction. Ovatodiolide did not produce an increase in micronucleated reticulocytes in the micronucleus test. The results revealed that the acute oral toxicity of ovatodiolide is over 1000 mg/kg b.w. in rats. Moreover, 10, 25 and 50 mg/kg b.w. of ovatodiolide did not cause a significant effect in rats. According to the results of the genotoxicity and oral toxicity studies in rats, ovatodiolide did not produce any adverse effects, and the tested doses can serve as clinical references.

Keywords: 28-day oral toxicity study; Anisomeles indica (L.) Kuntze; Genotoxicity; Ovatodiolide; Rats.

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Conflict of interest statement

The authors report no declarations of interest.

Figures

None
Graphical abstract
Fig. 1
Fig. 1
The chemical structure and ORTEP diagram of ovatodiolide.
Fig. 2
Fig. 2
Body weight changes of rats in the ovatodiolide single oral toxicity study.
Fig. 3
Fig. 3
Body weight changes of rats in the 28-day oral toxicity study of ovatodiolide.
See this image and copyright information in PMC

References

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