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. 2021 Sep 20;8(3):489-493.
doi: 10.5455/javar.2021.h538. eCollection 2021 Sep.

ITS1-PCR based identification of chicken Eimeria species in poultry litter from Mymensingh district, Bangladesh

Mohammad Zahangir Alam  1 , Anita Rani Dey  1 , Shanaz Parvin  1 , Shirin Akter  1 , Sharmin Aqter Rony  1
Affiliations

ITS1-PCR based identification of chicken Eimeria species in poultry litter from Mymensingh district, Bangladesh

Mohammad Zahangir Alam et al. J Adv Vet Anim Res. .

Abstract

Objectives: The purpose of this study was to determine the species composition of Eimeria circulating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay.

Materials and methods: Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichromate from litter slurry collected from 13 commercially active broiler farms in the research region. Genomic DNA was isolated from sporulated oocysts and used to amplify the Eimeria species-specific ITS1 gene by PCR amplification. Electrophoresis of 1.5% agarose gel was used to visualize the amplified PCR products.

Results: In the study samples from Mymensingh district, Bangladesh, the presence of Eimeria brunetti, Eimeria acervulina, Eimeria necatrix, Eimeria mitis, and Eimeria tenella was identified.

Conclusions: The findings of this study may shed light on the zonal approach to chicken coccidiosis control. Additionally, it suggests that ITS1-based PCR might be used in the field to accurately identify Eimeria species.

Keywords: Coccidiosis; Eimeria species; ITS1-PCR; chicken; identification.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.. Images showing the Internal transcribed spacer 1 (ITS1)-PCR products from oocyst samples resolved on 1.5% agarose gel. PCR amplification was found using primers specific for (a) E. brunetti (~310 bp), (b) E. acervulina (~321 bp), (c) E. necatrix (~285 bp) and (d) E. mitis (~306 bp), and (e) E. tenella (~271 bp). DNA ladder of 100 bp molecular weight was used as marker.
See this image and copyright information in PMC

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