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. 2022 Jan;49(1):293-302.
doi: 10.1007/s11033-021-06871-3. Epub 2021 Nov 1.

Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

Jazba Anum  1 , Charlotte O'Shea  2 , M Zeeshan Hyder  1 , Sumaira Farrukh  1 , Karen Skriver  2 , Saad Imran Malik  3 , Tayyaba Yasmin  4
Affiliations

Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

Jazba Anum et al. Mol Biol Rep. 2022 Jan.

Abstract

Background: Germin-like proteins (GLPs) are ubiquitous plant proteins, which play significant role in plant responses against various abiotic stresses. However, the potential functions of GLPs in rice (Oryza sativa) against salt and drought stress are still unclear.

Methods and results: In this study, transcriptional variation of eight OsGLP genes (OsGLP3-6, OsGLP4-1, OsGLP8-4, OsGLP8-7, OsGLP8-10, OsGLP8-11 and OsGLP8-12) was analyzed in leaves and roots of two economically important Indica rice cultivars, KS282 and Super Basmati, under salt and drought stress at early seedling stage. The relative expression analysis from qRT-PCR indicated the highest increase in expression of OsGLP3-6 in leaves and roots of both rice varieties with a significantly higher expression in KS282. Moreover, relative change in expression of OsGLP8-7, OsGLP8-10 and OsGLP8-11 under salt stress and OsGLP8-7 under drought stress was also commonly higher in leaves and roots of KS282 as compared to Super Basmati. Whereas, OsGLP3-7 and OsGLP8-12 after salt stress and OsGLP8-4 and OsGLP8-12 after drought stress were observed with higher relative expression in roots of Super Basmati than KS282. Importantly, the OsGLP3-6 and OsGLP4-1 from chromosome 3 and 4 respectively showed higher expression in leaves whereas most of the OsGLP genes from chromosome 8 exhibited higher expression in roots.

Conclusion: Overall, as a result of this comparative analysis, OsGLP genes showed both general and specific expression profiles depending upon a specific rice variety, stress condition as well as tissue type. These results will increase our understanding of role of OsGLP genes in rice crop and provide useful information for the further in-depth research on their regulatory mechanisms in response to these stress conditions.

Keywords: Abiotic stress; Expression profiling; Germin-like proteins; Oryza sativa.

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References

    1. Caliskan M (2009) Salt stress causes a shift in the localization pattern of germin gene expression. Genet Mol Res 8(4):1250–1256 - DOI
    1. Davidson RM, Reeves PA, Manosalva PM, Leach JE (2009) Germins: a diverse protein family important for crop improvement. Plant Sci 177(6):499–510 - DOI
    1. Dunwell JM, Gibbings JG, Mahmood T, Saqlan Naqvi SM (2008) Germin and germin-like proteins: evolution, structure, and function. CRC Crit Rev Plant Sci 27(5):342–375 - DOI
    1. Jiang Y, Yang B, Harris NS, Deyholos MK (2007) Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots. J Exp Bot 58(13):3591–3607 - DOI
    1. Lu M, Han YP, Gao JG, Wang XJ, Bin LW (2010) Identification and analysis of the germin-like gene family in soybean. BMC Genom. https://doi.org/10.1186/1471-2164-11-620 - DOI

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