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. 2021 Sep 30;26(3):346-356.
doi: 10.3746/pnf.2021.26.3.346.

An Extract and Fractions from Coffea arabica Sediment on Antioxidant and Anti-Tyrosinase Activities, and on the Quality of Whiteleg Shrimp (Litopenaus vannamei) during Refrigerated Storage

Dao Thi Anh Phan  1 , Hue Thi Ha  1 , Tinh Trung Ho  2
Affiliations

An Extract and Fractions from Coffea arabica Sediment on Antioxidant and Anti-Tyrosinase Activities, and on the Quality of Whiteleg Shrimp (Litopenaus vannamei) during Refrigerated Storage

Dao Thi Anh Phan et al. Prev Nutr Food Sci. .

Abstract

Coffea arabica sediment, a popular agro-by-product from the coffee juice processing industry, contains many phytochemicals, and it is often discarded as waste. The antioxidant and anti-tyrosinase activities of an ethanol extract and its active fractions obtained from Coffea arabica sediment was studied. Additionally, whiteleg shrimp (Litopenaeus vannamei) was treated with the extract and fractions, and the quality during refrigerated storage was evaluated. The most active fraction (P<0.05) fraction III from the Coffea arabica sediment extract showed a total phenolic content of 116.5 mg gallic acid equivalent/g, an inhibitory concentration of 50% value of 27.3 μg/mL in the 2,2-diphenyl-1-picryl hydrazyl test, an absorbance of 3.7 (at 1.0 μg/mL) in the reducing power assay, and 79.5% inhibition for the tyrosinase inhibition test (at 100 μg/mL). Whiteleg shrimp treated with PD-3 and crude extract (CE) retarded the growth of bacteria and spoilage microor-ganisms in comparison to the control (P<0.05). Lower values in pH, thiobarbituric acid reactive substances, and total volatile base content were enumerated in samples treated with PD-3 and CE (P<0.05) compared to controls. Loss in freshness, protein content, and melanosis formation declined with CE and PD-3 treatments. Our findings showed that Coffea arabica sediment has antioxidant and antimicrobial properties. Also, it could be a promising treatment in melanosis prevention, and a natural additive for extending the shelf life of whiteleg shrimp during refrigerated storage; thus an alternative to traditional sulfites.

Keywords: Coffea arabica; antioxidant; melanosis; tyrosinase; whiteleg shrimp (Litopenaus vannamei).

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Conflict of interest statement

AUTHOR DISCLOSURE STATEMENT The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Changes in pH of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Values represent the mean±standard deviation (n=3). Significant differences (P<05) among different days of storage in the same treatment are identified with different uppercase letters (A-H). Significant differences (P<05) among different treatments in the same storage time are identified with different lowercase letters (a-d). Control, sample treated water; SMS, sample treated with sodium metabisulfite (1.25%); CE, sample treated with Coffea arabica extract, PD-3, sample treated with PD-3 fraction obtained from Coffea arabica sediment extract.
Fig. 2
Fig. 2
Changes in the mean gray values of the carapace area of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Please see Fig. 1 for the caption and legends.
Fig. 3
Fig. 3
Changes in the TBARS values of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Please see Fig. 1 for the caption and legends.
Fig. 4
Fig. 4
Total plate (A) and Enterobacteriaceae count (B) of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Please see Fig. 1 for the caption and legends.
Fig. 5
Fig. 5
Protein content of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Please see Fig. 1 for the caption and legends.
Fig. 6
Fig. 6
Shear force of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Please see Fig. 1 for the caption and legends.
Fig. 7
Fig. 7
Total volatile base (TVB-N) content of whiteleg shrimp, Litopenaus vannamei during refrigerated storage. Please see Fig. 1 for the caption and legends.
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