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. 2021 Dec;12(2):10716-10728.
doi: 10.1080/21655979.2021.2001952.

Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p

Ning Jiang  1 , Lijun Zhao  1 , Dan Zong  1 , Li Yin  1 , Lirong Wu  1 , Cheng Chen  1 , Xue Song  1 , Qian Zhang  1 , Xuesong Jiang  1 , Xia He  1 , Jifeng Feng  2
Affiliations

Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p

Ning Jiang et al. Bioengineered. 2021 Dec.

Abstract

Nasopharyngeal carcinoma (NPC) is a typical type of malignant tumor. This research paper aims to study the function and mechanism of long non-coding RNA lung adenocarcinoma-related transcript 1 (lncRNA-LUADT1) in the progression of NPC. In this study, the expressions of lncRNA-LUADT1, miR-1207-5p, and TEAD1 in NPC tissues and cell lines were detected by RT-qPCR. Initially, the expression of lncRNA-LUADT1 and TEAD1 were significantly up-regulated in NPC tissues and cells, while miR-1207-5p was significantly down-regulated. Next, miR-1207-5p was confirmed to bind to lncRNA-LUADT1 or TEAD1 by bioinformatics and luciferase reporter assay. In addition, after interfering with lncRNA-LUADT1 expression, experiments of CCK8, EDU staining, and Transwell invasion were used to detect proliferation, invasion, and migration of NPC cells. The results showed that interfering with lncRNA-LUADT1 expression could inhibit the proliferation, invasion, and migration of NPC cells. Western blot showed that lncRNA-LUADT1 knockdown significantly decreased the expression of Hippo/YAP pathway protein (YAP1 and TAZ). However, interfering with the expression of miR-1207-5p reversed these results. In addition, the nude mouse tumor formation experiment suggested that low-expressed lncRNA-LUADT1 reduced the volume and weight of tumor tissues. In summary, lncRNA-LUADT1 down-regulation could inhibit NPC cell proliferation and invasion, which may be achieved through regulating miR-1207-5p expression and affecting TEAD1 expression, thus inhibiting the activation of Hippo/YAP signaling pathway.

Keywords: Hippo/YAP signaling pathway; Nasopharyngeal carcinoma; TEAD1; lncRNA-LUADT1; miR-1207-5p.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
lncRNA-LUADT1 expression levels in tissues and cells of nasopharyngeal carcinoma patients
Figure 2.
Figure 2.
The effect of LUADT1 expression on the nasopharyngeal carcinoma process
Figure 3.
Figure 3.
The targeting effect of lncRNA-LUADT1 on miR-1207-5p
Figure 4.
Figure 4.
TEAD1 is the target gene of miR-1207-5p
Figure 5.
Figure 5.
The effect of lncRNA-LUADT1 and miR-1207-5p on the nasopharyngeal carcinoma process
Figure 6.
Figure 6.
lncRNA-LUADT1 effect on tumor growth in nude mice
See this image and copyright information in PMC

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