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. 2021 Dec;12(2):10690-10702.
doi: 10.1080/21655979.2021.2001905.

N6-Methyladenosine-regulated LINC00675 suppress the proliferation, migration and invasion of breast cancer cells via inhibiting miR-513b-5p

Shenglan Fan  1 , Liping Wang  2
Affiliations

N6-Methyladenosine-regulated LINC00675 suppress the proliferation, migration and invasion of breast cancer cells via inhibiting miR-513b-5p

Shenglan Fan et al. Bioengineered. 2021 Dec.

Abstract

Breast cancer (BC) is the most common cancer among women. LINC00675 and miR-513b-5p has been reported to be abnormally expressed in multiple types of cancers and modulate malignant phenotypes of cancer cells. However, to date, the functional role and underlying regulatory mechanism of LINC00675 and miR-513b-5p in BC remains largely unknown. Here, we found that LINC00675 was significantly downregulated in BC tissues and cell lines. Decrease of LINC00675 expression associated with higher tumor grade, lymphovascular invasion and shorter survival in BC patients. Functional experiments demonstrated that overexpression of LINC00675 suppressed BC cell proliferation, migration and invasion, whereas depletion of LINC00675 exerted opposite effects. Mechanistically, LINC00675 functioned as a competing endogenous RNA (ceRNA) to interact with miR-513b-5p and suppress its expression. Moreover, METTL3 increased the m6A methylation of LINC00675, which enhanced the association between LINC00675 and miR-513b-5p. Collectively, the central findings of our study suggest that LINC00675 represses BC progression through the inhibition of miR-513b-5p in a m6A-dependent manner.

Keywords: METTL3; ceRNA; m6Amethylation; miR-513b-5p.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Downregulation of LINC00675 is associated with poor prognosis of BC patients
Figure 2.
Figure 2.
LINC00675 suppresses the proliferation, migration and invasion of BC cells
Figure 3.
Figure 3.
LINC00675 associates with miR-513b-5p
Figure 4.
Figure 4.
LINC00675 exerts suppressive effects via miR-513b-5p
Figure 5.
Figure 5.
m6A modification is involved in the ceRNA activity of LINC00675
Figure 6.
Figure 6.
The schematic diagram of proposed mechanism of LINC00675 regulating miR-513b-5p in BC cells
See this image and copyright information in PMC

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