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. 2021 Dec;11(6):651-658.
doi: 10.1007/s13659-021-00321-0. Epub 2021 Nov 5.

Furostanol Saponins from Asparagus cochinchinensis and Their Cytotoxicity

Ruo-Song Zhang  1   2 , Yang-Yang Liu  3 , Pei-Feng Zhu  1   2 , Qiong Jin  1   2 , Zhi Dai  3 , Xiao-Dong Luo  4   5
Affiliations

Furostanol Saponins from Asparagus cochinchinensis and Their Cytotoxicity

Ruo-Song Zhang et al. Nat Prod Bioprospect. 2021 Dec.

Abstract

Phytochemical investigation on the roots of Asparagus cochinchinensis led to the isolation of one new furostanol saponin, named 26-O-β-D-glucopyranosyl-22α-hydroxyl-(25R)-Δ5(6)-furost-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 4)]-β-D-glucopyranoside (1), along with three known congeners (2‒4). The structure of new saponin was elucidated via comprehensive inspection of its HRMS and NMR spectral data as well as chemical technology, whereas those of known ones were identified by comparison of their NMR and MS spectral data with those reported in literatures. All isolated saponins were evaluated for their cytotoxic effects on two human liver (MHCC97H) and lung adenocarcinoma (H1299) cancer cells in vitro. Among them, both 1 and 2 showed significant cytotoxicity against above mentioned cell lines. Further studies revealed that these two saponins could significantly inhibit their proliferation of MHCC97H and H1299 cells.

Keywords: Asparagus cochinchinensis; Cytotoxicity; Steroid saponins; Structural elucidation.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Fig. 1
Fig. 1
Structures of 1‒4
Fig. 2
Fig. 2
Key 1H ‒ 1H COSY and HMBC correlations of 1
Fig. 3
Fig. 3
Key ROESY correlations for the aglycone moiety of 1
Scheme 1
Scheme 1
The fragmentation process of 1 in the ESI- MS negative scan
Fig. 4
Fig. 4
Effects of 1 and 2 on MHCC97H and H1299 cells proliferation (n = 3). A The IC50 values of 1 and 2 against MHCC97H; B The IC50 values of 1 and 2 against H1299; C Inhibition effects of MHCC97H and H1299 cells proliferation by 1 and 2 after cultivation for 72 h
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