Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Mar;5(3):879-92.
doi: 10.1093/nar/5.3.879.

The effect of specific structural modification on the biological activity of E. coli arginine tRNA

T A Kruse, B F Clark
Free PMC article

The effect of specific structural modification on the biological activity of E. coli arginine tRNA

T A Kruse et al. Nucleic Acids Res. 1978 Mar.
Free PMC article

Abstract

Escherichia coli arginine tRNA1 has been modified at position s2C32 with iodoacetamide and a spin labelled derivative. The small effects on the charging ability of tRNA by the modifiications suggest that the synthetase does not bind to the tRNA in this region of the anticodon loop before the anticodon. A ternary complex of elongation factor Tu, GTP and the modified Arg-tRNA, can be formed allowing future studies of enzymatic binding to the ribosome. Using the triplet binding assay the native Arg-tRNA1 decodes all 4 codons beginning with CG. The modified Arg-tRNA1 has a restricted decoding but the decoding pattern is still unusual according to the Wobble Hypothesis.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1976 Jun 7;70(3):1009-13 - PubMed
    1. Science. 1976 Nov 19;194(4267):796-806 - PubMed
    1. Nucleic Acids Res. 1977 Jun;4(6):1999-2008 - PubMed
    1. Biochim Biophys Acta. 1970 Apr 15;204(2):620-3 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1010-7 - PubMed