Development of chitosan/poly (vinyl alcohol)/graphene oxide loaded with vanadium doped titanium dioxide patch for visible light driven antibacterial activity and accelerated wound healing application

Abstract

Wound healing is a multi-stage process that is dynamic, interactive, and complicated. However, many nanomaterials are employed to expedite wound healing by demonstrating antibacterial activity or boosting cell proliferation. But only one phase is focused during the wound healing process. As a result, there is a need for optimum wound dressing materials that promotes different wound healing cascades with ideal properties. Herein, Graphene Oxide loaded with vanadium (V) doped titanium dioxide (TiO₂) blended with chitosan, and polyvinyl alcohol (CS/PVA/GO/TiO₂-V) patch was developed for wound healing. XRD, FTIR and FE-SEM analyses were carried out to study the morphology and structural property of the patch. The fabricated patch has a high surface porosity, excellent moisture vapor transfer rate, appropriate swelling behaviour, and oxygen permeability, which results in an excellent moist environment for wound breathing and effective management of wound exudates. The antibacterial test showed significant antibacterial efficacy against wound infections in the presence of light when compared to dark. In-vitro analysis such as hemocompatibility, cytotoxicity, cell adhesion, and scratch assay show the predicted potential wound healing application with high biocompatibility. These results suggest that CS/PVA/GO/TiO₂-V patch provides a microenvironment favourable to cells' growth and differentiation and positively modulates full-thickness wounds' healing.

Keywords: Biocompatibility; Chitosan; Graphene oxide-titanium dioxide; Photocatalytic; Wound healing.