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. 2021 Oct 29:14:4511-4516.
doi: 10.2147/IDR.S324055. eCollection 2021.

Intensive Poultry Farming Practices Influence Antibiotic Resistance Profiles in Pseudomonas aeruginosa Inhabiting Nearby Soils

Shaheena Kousar  1 , Nikhar Rehman  1 , Asghar Javed  2 , Aamir Hussain  3 , Muhammad Naeem  1 , Samra Masood  1 , Hafiz Arslan Ali  1 , Amna Manzoor  1 , Aleem Ahmed Khan  1 , Ahmed Akrem  1 , Fatima Iqbal  4 , Aqsa Zulfiqar  1 , Muhammad Bilal Jamshaid  1 , Muhammad Waqas  1 , Asma Waseem  1 , Muhammad Qamar Saeed  1
Affiliations

Intensive Poultry Farming Practices Influence Antibiotic Resistance Profiles in Pseudomonas aeruginosa Inhabiting Nearby Soils

Shaheena Kousar et al. Infect Drug Resist. .

Abstract

Background: The drastic increase in use of antibiotics as a mandatory part of production in poultry and livestock has led to the development of bacterial resistance against antibiotics. The spread of resistant bacteria from poultry to humans increases the risk of treatment failure by antibiotics because of resistance genes transfer.

Study objective: The objective of the study was to estimate and compare the P. aeruginosa resistance profile collected from areas around the poultry farm premises and areas at least 500 meters away from the nearest poultry farm. We studied the effect of antibiotic usage in farms on the bacterial profile present in the upper layer of soil.

Methodology: A total of 1,200 moist soil samples were collected from areas within a 25 meters range of poultry farms and areas that had no poultry farms in its 500 meters vicinity. P. aeruginosa was cultured and isolated. The antibiotic susceptibility profile was carried out by Kirby-Bauer disc diffusion method and results were analyzed according to CLSI guidelines. Statistical analysis was carried out to check the significance of results.

Results: A total of 300 P. aeruginosa isolates were isolated, among which 140 isolates were isolated from areas around the poultry farm premises and had higher prevalence of antibiotic resistance. A total of 160 isolates were isolated from areas outside the poultry farm range. Resistance was not as high as in the isolates from around the farm. The ESBL production was higher in the isolates that were in close contact with the poultry farm as compared to the isolates away from the farm.

Conclusion: Use of antibiotics in the poultry farm for production significantly increases the resistance in bacterial strains present in the upper layer of soil around the poultry farm within at least a 25 meter range.

Keywords: MDR; P. aeruginosa; antibiotic resistance; poultry.

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Conflict of interest statement

The authors declare no conflicts of interest in this work.

References

    1. Aarestrup FM. Monitoring of antimicrobial resistance among food animals: principles and limitations. J Vet Med B. 2004;51(8–9):380–388. doi:10.1111/j.1439-0450.2004.00775.x - DOI - PubMed
    1. Aarestrup FM. Association between the consumption of antimicrobial agents in animal husbandry and the occurrence of resistant bacteria among food animals. Int J Antimicrob Agents. 1999;12(4):279–285. doi:10.1016/s0924-8579(99)90059-6 - DOI - PubMed
    1. Murphy D, Ricci A, Auce Z, et al. EMA and EFSA joint scientific opinion on measures to reduce the need to use antimicrobial agents in animal husbandry in the European Union, and the resulting impacts on food safety (RONAFA). EFSA J. 2017;15:1. doi:10.2903/j.efsa.2017.4666 - DOI - PMC - PubMed
    1. Manyi-Loh C, Mamphweli S, Meyer E, Okoh A. Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules. 2018;23(4). doi:10.3390/molecules23040795 - DOI - PMC - PubMed
    1. Aarestrup FM. Veterinary drug usage and antimicrobial resistance in bacteria of animal origin. Basic Clin Pharmacol Toxicol. 2005;96(4):271–281. doi:10.1111/j.1742-7843.2005.pto960401.x - DOI - PubMed

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