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. 2021 Oct 27:2021:9416794.
doi: 10.1155/2021/9416794. eCollection 2021.

The Preventive Effect of Lactobacillus plantarum ZS62 on DSS-Induced IBD by Regulating Oxidative Stress and the Immune Response

Yanni Pan  1   2 , Yujing Ning  3 , Jing Hu  1 , Zhiying Wang  4 , Xiufeng Chen  5 , Xin Zhao  1
Affiliations

The Preventive Effect of Lactobacillus plantarum ZS62 on DSS-Induced IBD by Regulating Oxidative Stress and the Immune Response

Yanni Pan et al. Oxid Med Cell Longev. .

Abstract

In this study, we used DSS to establish an IBD mouse model to study the preventive effect of Lactobacillus plantarum (L. plantarum) ZS62 on IBD in the context of oxidative stress and the immune response. We assessed the mitigating effect of this strain on IBD mice by examining the length of and histopathological changes in the colon, determining the serum antioxidant index and the levels of inflammatory cytokines, as well as the mRNA and protein expression levels of relevant genes. The study results showed that L. plantarum ZS62 could inhibit colonic atrophy in IBD mice, reduce the degree of colonic damage, downregulate the serum levels of MDA, MPO, IL-1β, IL-6, IL-12, TNF-α, and IFN-γ and the relative mRNA and protein expression of IL-1β, IL-12, TNF-α, COX-2, iNOS, and NF-κB p65 in mouse colon tissues, and upregulate the serum levels of CAT, T-SOD, and IL-10 and the relative mRNA and protein expression of Cu/Zn SOD, Mn SOD, GSH-Px, CAT, IL-10, and IκB-α in colon tissues. In summary, L. plantarum ZS62 exhibited a good preventive effect on DSS-induced IBD by regulating oxidative stress and the immune response.

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Conflict of interest statement

The authors declare no conflict of interest in this article.

Figures

Figure 1
Figure 1
Morphological characteristics of experimental lactic acid bacterium Lactobacillus plantarum ZS62.
Figure 2
Figure 2
Colon length (cm) of experimental mice. (a) Average length of experimental mouse colon. (b) Photo of mouse colon length. Normal: mice fed a standard chow diet plus drinking water; DSS: mice fed the standard chow diet plus drinking water with 5% dextran sulfate sodium; SSZ: sulfasalazine (500 mg/kg of BW) plus 5% DSS; ZS62: Lactobacillus plantarum ZS62 (1.0 × 109 CFU/mL) plus 5% DSS; LB: Lactobacillus bulgaricus (1.0 × 109 CFU/mL) plus 5% DSS. a–cMean values with different letters in the same bars are significantly different (p < 0.05) according to Duncan's new multiple range test (MRT).
Figure 3
Figure 3
Histopathological observation of colon tissues. Magnification 100x. Normal: mice fed a standard chow diet plus drinking water; DSS: mice fed the standard chow diet plus drinking water with 5% dextran sulfate sodium; SSZ: sulfasalazine (500 mg/kg of BW) plus 5% DSS; ZS62: Lactobacillus plantarum ZS62 (1.0 × 109 CFU/mL) plus 5% DSS; LB: Lactobacillus bulgaricus (1.0 × 109 CFU/mL) plus 5% DSS.
Figure 4
Figure 4
Concentrations of cytokines IL-6, IL-10, IL-12, TNF-α, and IFN-γ. (a–e) Mean values with different letters in the same column differ significantly (p < 0.05) by Duncan's multiple range test. Values presented are the means ± standard deviation (N = 10/group). Normal: mice fed a standard chow diet plus drinking water; DSS: mice fed the standard chow diet plus drinking water with 5% dextran sulfate sodium; SSZ: sulfasalazine (500 mg/kg of BW) plus 5% DSS; ZS62: Lactobacillus plantarum ZS62 (1.0 × 109 CFU/mL) plus 5% DSS; LB: Lactobacillus bulgaricus (1.0 × 109 CFU/mL) plus 5% DSS.
Figure 5
Figure 5
The mRNA and protein expression levels of Cu/Zn SOD, Mn SOD, GSH-Px, and CAT in mouse colon tissue. a–eMean values with different letters in the same column differ significantly (p < 0.05) by Duncan's multiple range test. Values presented are the means ± standard deviation (N = 10/group). Normal: mice fed a standard chow diet plus drinking water; DSS: mice fed the standard chow diet plus drinking water with 5% dextran sulfate sodium; SSZ: sulfasalazine (500 mg/kg of BW) plus 5% DSS; ZS62: Lactobacillus plantarum ZS62 (1.0 × 109 CFU/mL) plus 5% DSS; LB: Lactobacillus bulgaricus (1.0 × 109 CFU/mL) plus 5% DSS.
Figure 6
Figure 6
The mRNA and protein expression levels of IL-1β, IL-12, TNF-α, and IL-10 in mouse colon tissue. a–eMean values with different letters in the same column differ significantly (p < 0.05) by Duncan's multiple range test. Values presented are the means ± standard deviation (N = 10/group). Normal: mice fed a standard chow diet plus drinking water; DSS: mice fed the standard chow diet plus drinking water with 5% dextran sulfate sodium; SSZ: sulfasalazine (500 mg/kg of BW) plus 5% DSS; ZS62: Lactobacillus plantarum ZS62 (1.0 × 109 CFU/mL) plus 5% DSS; LB: Lactobacillus bulgaricus (1.0 × 109 CFU/mL) plus 5% DSS.
Figure 7
Figure 7
The mRNA and protein expression levels of COX-2, iNOS, NF-κB p65, and IκB-α in mouse colon tissue. a–eMean values with different letters in the same column differ significantly (p < 0.05) by Duncan's multiple range test. Values presented are the means ± standard deviation (N = 10/group). Normal: mice fed a standard chow diet plus drinking water; DSS: mice fed the standard chow diet plus drinking water with 5% dextran sulfate sodium; SSZ: sulfasalazine (500 mg/kg of BW) plus 5% DSS; ZS62: Lactobacillus plantarum ZS62 (1.0 × 109 CFU/mL) plus 5% DSS; LB: Lactobacillus bulgaricus (1.0 × 109 CFU/mL) plus 5% DSS.
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