Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jan-Feb;12(1):33-37.
doi: 10.1016/j.jobcr.2021.10.003. Epub 2021 Oct 8.

Evaluation of "soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), interleukin-1β, and matrix metalloproteinase-8" as a short panel of salivary biomarkers in patients with and without stage III/IV periodontitis and type 2 diabetes mellitus

Saranya Balaji  1 , Priyanka K Cholan  1 , Dhayanand John Victor  1
Affiliations

Evaluation of "soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), interleukin-1β, and matrix metalloproteinase-8" as a short panel of salivary biomarkers in patients with and without stage III/IV periodontitis and type 2 diabetes mellitus

Saranya Balaji et al. J Oral Biol Craniofac Res. 2022 Jan-Feb.

Abstract

Background and aim: Periodontitis involves a dynamic disease process, demanding the identification of biomarkers to diagnose the current state of disease activity. Therefore this study assessed the potential of "sTREM-1, IL-1β, and MMP-8" as a short panel of biomarkers of host biological process indicating the inflammatory burden in periodontium and thereby serving as a panel of diagnostic markers in periodontal disease.

Methods: Sixty eight patients were recruited and allotted into four groups comprising of subjects with clinically healthy gingiva and Stage III/IV Periodontitis with and without type 2 diabetes with HbA1c levels in the range of 6.5-7.9%. Periodontal parameters were measured and full mouth radiographic assessment was done. Whole saliva (unstimulated) samples were collected from all patients and estimation of the levels of markers was done employing ELISA.

Results: All the three biomarkers were noted to be the lowest in group I (sTREM-1: 75.63 ± 13.77; IL-1β: 15.67 ± 3.39; MMP-8: 85.83 ± 22.32) and highest in group IV (sTREM-1: 138.83 ± 14.89; IL-1β: 39.19 ± 7.20; MMP-8: 201.15 ± 50.32) with statistically significant difference. The difference observed between groups II and III for all the biomarkers assessed were statistically insignificant. The clinical parameters and HbA1c levels had positive correlation with the levels of biomarkers which was statistically significant.

Conclusion: This study unveils the potential of the short panel of biomarkers ("sTREM-1, IL-1β, and MMP-8") to be used as diagnostic and possible prognostic markers for Periodontitis. It further corroborates the role of type 2 diabetes mellitus in amplifying the diverse processes that result in periodontal destruction.

Keywords: Biomarkers; Diabetes; IL-1ß; MMP-8; Periodontal disease; Periodontitis-systemic interactions; sTREM-1.

PubMed Disclaimer

Conflict of interest statement

None.

Figures

Fig. 1
Fig. 1
Scatter plot representing correlation between biomarkers in all groups.
See this image and copyright information in PMC

References

    1. Hasturk H., Kantarci A. Activation and resolution of periodontal inflammation and its systemic impact. Periodontol. 2000 2015;69(1):255–273. doi: 10.1111/prd.12105. - DOI - PMC - PubMed
    1. Tessarz A.S., Cerwenka A. The TREM-1/DAP12 pathway. Immunol Lett. 2008;116(2):111–116. doi: 10.1016/j.imlet.2007.11.021. - DOI - PubMed
    1. Arts R.J., Joosten L.A.B., van der Meer JWM & Netea. MG TREM-1: intracellular signaling pathways and interaction with pattern recognition receptors. J Leukoc Biol. 2013;93:209–215. doi: 10.1189/jlb.0312145. - DOI - PubMed
    1. Baruah S., Keck K., Vrenios M. Identification of a novel splice variant isoform of TREM-1 in human neutrophil granules. J Immunol. 2015;195(12):5725–5731. doi: 10.4049/jimmunol.1402713. - DOI - PMC - PubMed
    1. Gómez-Piña V., Soares-Schanoski A., Rodríguez-Rojas A. Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes. J Immunol. 2007;179(6):4065–4073. doi: 10.4049/jimmunol.179.6.4065. - DOI - PubMed