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. 1987 Jul;84(13):4445-9.
doi: 10.1073/pnas.84.13.4445.

Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumor antibiotic of Streptomyces glaucescens

H Motamedi, C R Hutchinson

Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumor antibiotic of Streptomyces glaucescens

H Motamedi et al. Proc Natl Acad Sci U S A. 1987 Jul.

Abstract

Through complementation of mutations specifically blocking the biosynthesis of tetracenomycin C by Streptomyces glaucescens and selecting for resistance to tetracenomycin C in Streptomyces lividans, all of the genes for the production of tetracenomycin C were inserted in pIJ702, a high copy-number Streptomyces gene cloning vector. The tcm biosynthetic and resistance genes occur as a single cluster in the S. glaucescens genome and are expressed in heterologous streptomycete hosts like S. lividans, resulting in the overproduction of pigmented intermediates of the tetracenomycin C biosynthetic pathway.

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References

    1. J Gen Microbiol. 1974 Apr;81(2):463-74 - PubMed
    1. Nature. 1987 Feb 26-Mar 4;325(6107):818-21 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Arch Microbiol. 1979 May;121(2):111-6 - PubMed
    1. J Gen Microbiol. 1979 Sep;114(1):35-43 - PubMed

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