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. 1987 Jul;84(14):4841-5.
doi: 10.1073/pnas.84.14.4841.

Metal-dependent folding of a single zinc finger from transcription factor IIIA

A D Frankel, J M Berg, C O Pabo

Metal-dependent folding of a single zinc finger from transcription factor IIIA

A D Frankel et al. Proc Natl Acad Sci U S A. 1987 Jul.

Abstract

A 30-amino acid peptide, which corresponds to the second "zinc finger" domain of transcription factor IIIA, has been synthesized and purified. This peptide folds in the presence of zinc: adding Zn2+ significantly changes the circular dichroism spectrum, and Zn2+ protects the peptide from tryptic digestion. The peptide also binds Co2+, and the absorption spectrum of the Co2+ complex suggests that a tetrahedral binding site is formed by two cysteines and two histidines. Experiments at higher temperatures (60-75 degrees C) suggest that these folded metal-peptide complexes are quite thermostable. The peptide shows some sequence-specific effects in DNase and methylation protection experiments. However, it does not give a clear "footprint," and some effects are observed in the absence of added zinc.

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References

    1. Nature. 1978 Feb 9;271(5645):501 - PubMed
    1. J Mol Biol. 1970 Oct 14;53(1):159-62 - PubMed
    1. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 - PubMed
    1. Cell. 1980 Jan;19(1):13-25 - PubMed
    1. Cell. 1980 Jan;19(1):27-35 - PubMed

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