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. 2022 Feb;54(1):e14310.
doi: 10.1111/and.14310. Epub 2021 Nov 8.

Epigallocatechin 3-gallate improves the quality of bull semen cryopreservation

Zhiqiang Li  1   2 , Hongtao Wang  1   2 , Chongshan Yuan  1   2 , Ping Lu  1   2 , Yan Zhou  1   2 , Wenfa Lu  1   2 , Jing Zhao  1   2 , Hongyu Liu  1   2 , Jun Wang  1   2
Affiliations

Epigallocatechin 3-gallate improves the quality of bull semen cryopreservation

Zhiqiang Li et al. Andrologia. 2022 Feb.

Abstract

To determine the effects of epigallocatechin-3-gallate (EGCG) on the cryopreservation of bovine semen, epigallocatechin-3-gallate dissolved with double distilled water to 0.2, 0.4, 0.6 and 0.8 mg/ml were added to the cryopreservation diluent of the bull semen. Then, we used computer-assisted analysis of semen kinematic parameters, staining method to detect membrane function, acrosome integrity, enzyme-linked immunosorbent assay to detect catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehydes (MDA) and reactive oxygen levels. The results showed that adding 0.6 mg/L of epigallocatechin-3-gallate could improve the cryopreserved sperm quality, which significantly increased the total motility, distance average path, distance straight line, distance curved line, average path velocity, curvilinear-velocity, straight-line velocity, amplitude of lateral head displacement and beat/cross frequency, as well as sperm CAT, GSH-Px and SOD levels (p < 0.05), whilst reducing the reactive oxygen species and MDA levels (p < 0.05). Hence, these results indicate that the addition of 0.6 mg/ml of EGCG has a protective effect on the cryopreservation of the bovine semen.

Keywords: bull semen; cryopreservation; epigallocatechin-3-gallate; sperm analysis.

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