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. 2022 Feb;37(2):310-321.
doi: 10.1002/tox.23399. Epub 2021 Nov 9.

The biomechanistic aspects of renal cortical injury induced by diesel exhaust particles in rats and the renoprotective contribution of quercetin pretreatment: Histological and biochemical study

Ahmed A Morsi  1 , Hanan Fouad  2   3 , Wardah Abdullah Alasmari  4 , Eman Mohamed Faruk  4   5
Affiliations

The biomechanistic aspects of renal cortical injury induced by diesel exhaust particles in rats and the renoprotective contribution of quercetin pretreatment: Histological and biochemical study

Ahmed A Morsi et al. Environ Toxicol. 2022 Feb.

Erratum in

Abstract

Although several studies have reported a toxic effect of diesel exhaust particles (DEP) exposure on the kidney tissues, the involvement of autophagy/NF-kB signaling as encountered mechanisms and the protective effects of a natural flavonoid, quercetin on DEP remains unclear. Thirty-two albino rats were divided as control, quercetin-treated (60 mg/kg, oral), DEP-exposed (0.5 mg/kg, intra-tracheal), and quercetin/DEP-exposed groups. Specimens of the renal cortex were subjected to histo-biochemical study and immunohistochemical analysis using anti-NF-kB, and anti-LC3β antibodies followed by morphometric and statistical analyses. The expression level of autophagy genes was quantitatively evaluated using RT-PCR, as well. The DEP-exposed rats showed an elevation in the renal tissue levels of MDA and a decrease in the catalase and superoxide dismutase (p < .05). Histologically, there were cytoplasmic vacuolar changes in the lining cells of the renal tubules, glomerular atrophy, and vascular congestion. In addition, renal inflammation was evident as confirmed by the increased NF-kB immunoexpression. Moreover, the gene expression of Becn1, ATG5, and LC3β increased (p <. 0) due to DEP exposure. Conversely, quercetin pretreatment improved these renal histo-biochemical alterations (p < .05) and regulated autophagy/NF-kB pathways. Overall, the study proved the renal toxicity mediated by DEP exposure via precipitating renal inflammation, autophagy activation, and oxidative stress. Quercetin pretreatment could antagonize such machinery to protect the kidney against DEP.

Keywords: NF-kB; air pollution; autophagy; immunohistochemistry; oxidative stress; rat kidney.

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References

REFERENCES

    1. Manisalidis I, Stavropoulou E, Stavropoulos A, Bezirtzoglou E. Environmental and health impacts of air pollution: a review. Front Public Health. 2020;20(8):14. https://pubmed.ncbi.nlm.nih.gov/32154200
    1. de Jesus AL, Rahman MM, Mazaheri M, et al. Ultrafine particles and PM2.5 in the air of cities around the world: are they representative of each other? Environ Int. 2019;129:118-135. https://www.sciencedirect.com/science/article/pii/S0160412019311110
    1. Klein SG, Cambier S, Hennen J, et al. Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter. Part Fibre Toxicol. 2017;14(1):1-17.
    1. Nemmar A, Al-Salam S, Beegam S, Yuvaraju P, Hamadi N, Ali BH. In vivo protective effects of nootkatone against particles-induced lung injury caused by diesel exhaust is mediated via the NF-κB pathway. Nutrients. 2018;10(3):263.
    1. Nemmar A, Karaca T, Beegam S, et al. Prolonged pulmonary exposure to diesel exhaust particles exacerbates renal oxidative stress, inflammation and DNA damage in mice with adenine-induced chronic renal failure. Cell Physiol Biochem. 2016;38(5):1703-1713.

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