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. 2021 Oct 31:2021:7011493.
doi: 10.1155/2021/7011493. eCollection 2021.

Chemical Composition and In Vitro Antioxidant and Antimicrobial Activities of Marrubium vulgare L

Ibrahim Mssillou  1 , Abdelkrim Agour  1 , Noureddine Hamamouch  2 , Badiaa Lyoussi  1 , Elhoussine Derwich  1   3
Affiliations

Chemical Composition and In Vitro Antioxidant and Antimicrobial Activities of Marrubium vulgare L

Ibrahim Mssillou et al. ScientificWorldJournal. .

Abstract

In this study, the polyphenol content and the antioxidant and antimicrobial activities of hydroethanolic (MVE) and hydroacetonic (MVA) leaf extracts of Marrubium vulgare L. were examined. The results indicated that the total phenolic content was higher in MVA (112.09 ± 4.77 mg GAE/DW) compared to MVE extract (98.77 ± 1.68 mg GAE/DW). The total flavonoid content was also higher in MVA extract (21.08 ± 0.38 mg QE/g DW) compared to MVE (17.65 ± 0.73 mg QE/g DW). Analysis of the chemical composition revealed the presence of 13 compounds with a total of 96.14%, with the major compound being malic acid (22.57%). Both extracts possess a good total antioxidant activity. DPPH and FRAP assays indicated that the MVE extract possesses a better antioxidant activity, with IC50 = 52.04 µg/mL ± 0.2 and EC50 of 4.51 ± 0.5 mg/mL, compared to MVA extract (IC50 = 60.57 ± 0.6 µg/mL and EC50 of 6.43 ± 0.0411 mg/mL). Moreover, both extracts exhibited strong antimicrobial activity against certain nosocomial strains as indicted by the MIC values, which ranged between 0.93 mg/mL and 10 mg/mL. Taken together, these results reveal the importance of M. vulgare as a natural antioxidant with important antimicrobial activity.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
TPC, TFC, and TTC in mg (gallic acid, quercetin, and ascorbic acid, respectively) equivalent/gram dry weight of two extracts from Marrubium vulgare L. The results are expressed by means ± standard deviation, and values with different letters are significantly different (P < 0.05).
See this image and copyright information in PMC

References

    1. Karuppiah P., Mustaffa M. Antibacterial and antioxidant activities of musa sp. Leaf extracts against multidrug resistant clinical pathogens causing nosocomial infection. Asian Pacific Journal of Tropical Biomedicine . 2013;3(9):737–742. doi: 10.1016/s2221-1691(13)60148-3. - DOI - PMC - PubMed
    1. Abirami S., Priyalakshmi M., Soundariya A., et al. Antimicrobial activity, antiproliferative activity, amylase inhibitory activity and phytochemical analysis of ethanol extract of corn (zea mays L.) silk. Current Research in Green and Sustainable Chemistry . 2021;4 doi: 10.1016/j.crgsc.2021.100089.100089 - DOI
    1. Nurcholis W., Sya’bani Putri D. N., Husnawati H., Aisyah S. I., Priosoeryanto B. P. Total flavonoid content and antioxidant activity of ethanol and ethyl acetate extracts from accessions of amomum compactum fruits. Annals of Agricultural Science . 2021;66(1):58–62. doi: 10.1016/j.aoas.2021.04.001. - DOI
    1. Labiad H., Et-tahir A., Ghanmi M., et al. Ethnopharmacological survey of aromatic and medicinal plants of the pharmacopoeia of northern Morocco. Ethnobotany Research and Applications . 2020;19:1–16. doi: 10.32859/era.19.45.1-16. - DOI
    1. Hamamouch N. Use of ethnomedicinal plants by the people living in the middle atlas mountains in Morocco. Medicinal & Aromatic Plants . 2020;9 doi: 10.35248/2167-0412.20.9.349. - DOI

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