Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Aug;84(16):5670-4.
doi: 10.1073/pnas.84.16.5670.

Thyroid hormone receptor binds to a site in the rat growth hormone promoter required for induction by thyroid hormone

R J Koenig, G A Brent, R L Warne, P R Larsen, D D Moore

Thyroid hormone receptor binds to a site in the rat growth hormone promoter required for induction by thyroid hormone

R J Koenig et al. Proc Natl Acad Sci U S A. 1987 Aug.

Abstract

Transcription of the rat growth hormone (rGH) gene in pituitary cells is increased by addition of thyroid hormone (T3). This induction is dependent on the presence of specific sequences just upstream of the rGH promoter. We have partially purified T3 receptor from rat liver and examined its interaction with these rGH sequences. We show here that T3 receptor binds specifically to a site just upstream of the basal rGH promoter. This binding site includes two copies of a 7-base-pair direct repeat, the centers of which are separated by 10 base pairs. Deletions that specifically remove the T3 receptor binding site drastically reduce response to T3 in transient transfection experiments. These results demonstrate that T3 receptor can recognize specific DNA sequences and suggest that it can act directly as a positive transcriptional regulatory factor.

PubMed Disclaimer

References

    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9021-5 - PubMed
    1. Cell. 1980 Jun;20(2):269-81 - PubMed
    1. Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 - PubMed
    1. Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 - PubMed

Publication types

LinkOut - more resources