Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2386:17-26.
doi: 10.1007/978-1-0716-1771-7_2.

Indirect Immunofluorescence of Tissue Sections

Cody J Aros  1   2   3
Affiliations

Indirect Immunofluorescence of Tissue Sections

Cody J Aros. Methods Mol Biol. 2022.

Abstract

Immunofluorescence (IF) on tissue sections allows for the detection of protein species subcellular localization. IF studies further offer the ability to achieve this understanding at the level of single cell granularity. Here, we describe the processes by which tissue is fixed, embedded, sectioned, and subsequently utilized for conducting indirect IF assays. We raise potential opportunities for troubleshooting and optimization at varying stages of the protocol.

Keywords: Antibody; Antigen retrieval; Blocking; Immunofluorescence; Permeabilization.

PubMed Disclaimer

References

    1. Odell ID, Cook D (2013) Immunofluorescence techniques. J Invest Dermatol 133(1):1–4 - DOI
    1. Aoki V, Sousa JX, Fukumori LMI, Périgo AM, Freitas EL, Oliveira ZNP (2010) Direct and indirect immunofluorescence. An Bras Dermatol 85(4):490–500 - DOI
    1. Im K, Mareninov S, Diaz MFP, Yong WH (2019) An introduction to performing immunofluorescence staining. Methods Mol Biol 1897:299–311 - DOI
    1. Hewitson TD, Wigg B, Becker GJ (2010) Tissue preparation for histochemistry: fixation, embedding, and antigen retrieval for light microscopy. Methods Mol Biol 611:3–18 - DOI

MeSH terms

LinkOut - more resources