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. 2022:2383:569-578.
doi: 10.1007/978-1-0716-1752-6_36.

DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent

Kristi Kasemaa  1 , Kaia Talts  1 , Evelin Täht  1 , Urve Toots  2 , Mart Ustav  1
Affiliations

DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent

Kristi Kasemaa et al. Methods Mol Biol. 2022.

Abstract

Mammalian transient expression systems enable flexible and rapid production of proteins. They're ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more native folding and post-translational modifications-such as glycosylation-than expression systems based on hosts such as E. coli, yeast, or insect cells.Here we describe transient protein production using QMCF Technology (Icosagen, Estonia) that uses specific suspension-adapted mammalian cell lines (QMCF cells), appropriate QMCF episomal expression vector, a chemically defined animal origin-free, serum-free medium, and Reagent 007™ (Icosagen, Estonia) for efficient delivery of nucleic acids for transfection of mammalian cells in comparison with PEI MAX™ (Transfection Grade Linear Polyethylenimine Hydrochloride, Polysciences).

Keywords: Cell culture; QMCF technology; Recombinant protein production; Transfection reagent R007.

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