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. 2021 Oct 22;22(21):11410.
doi: 10.3390/ijms222111410.

Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders

Jolanta Lis-Kuberka  1 , Paulina Kubik  2 , Agnieszka Chrobak  2   3 , Jarosław Pająk  3 , Anna Chełmońska-Soyta  2 , Magdalena Orczyk-Pawiłowicz  1
Affiliations

Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders

Jolanta Lis-Kuberka et al. Int J Mol Sci. .

Abstract

The diagnosis of endometriosis and fertility disorders is difficult; therefore, it is necessary to look for reliable biomarkers. Analysis of the molecular status of fibronectin as a key player in repair and wound healing processes, as well as in coagulation and fibrinolysis pathways, is justified. ELISA and SDS-agarose immunoblotting were applied to determine the fibronectin concentration and presence and occurrence of soluble FN-fibrin complexes in the blood plasma of women with endometriosis (n = 38), fertility disorders (n = 28) and the healthy group (n = 25). The concentration of fibronectin in the blood plasma of women with endometriosis (292.61 ± 96.17 mg/L) and fertility disorders (287.53 ± 122.68 mg/L) was significantly higher than in the normal group (226.55 ± 91.98 mg/L). The presence of FN-fibrin complexes of 750, 1000, 1300, 1600 and 1900 kDa in the plasma of women with endometriosis and fertility disorders was shown. The presence of FN-fibrin complexes with a molecular mass of more than 1300 kDa in women with endometriosis and infertility and the complete absence of these complexes in healthy women may indicate an increased and chronic activation of coagulation mechanisms in these patients. The presence of complexes of high molecular mass may be one of the biomarkers of fertility disorders in women.

Keywords: endometriosis; fertility disorders; fibrin; molecular status of fibronectin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Flow chart of the study.
Figure 2
Figure 2
Box plot illustrating the FN concentrations in women’s plasma with endometriosis, fertility disorders and normal groups. The FN concentration were determined as described in Materials and Methods. Data are given as mean values, median and (25th and 75th) quartiles.
Figure 3
Figure 3
Representative immunopatterns of FN-fibrin complexes in blood plasma of women with endometriosis, fertility disorders and in the normal group.The 66 blood plasma samples of women with endometriosis and fertility disorders and 25 plasma samples from healthy individuals were subjected to SDS-agarose immunoblotting under non-reducing conditions [50,55]. Samples: Lanes 1–4 plasma of women with endometriosis; lanes 5–7 plasma of women with fertility disorders; lanes 8–10 plasma of women from control group. The molecular masses of plasma FN-fibrin complexes of 750 to 1900 kDa and 500 kDa FN dimer are shown by arrows on the left.
Figure 4
Figure 4
Receiver operating characteristic (ROC) curves for blood plasma FN concentration, FN dimer 500 kDa and FN-fibrin complex 750 kDa as markers of endometriosis (A–C) and fertility disorders (D–F). Data are given as area under the ROC curve (AUC) with a 95% confidence interval.
See this image and copyright information in PMC

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