Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Published Erratum
. 2021 Dec 1;41(48):10031-10032.
doi: 10.1523/JNEUROSCI.2028-21.2021. Epub 2021 Nov 12.

Erratum: Barrientos et al., "Axonal Degeneration Is Mediated by the Mitochondrial Permeability Transition Pore"

No authors listed
Published Erratum

Erratum: Barrientos et al., "Axonal Degeneration Is Mediated by the Mitochondrial Permeability Transition Pore"

No authors listed. J Neurosci. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Pharmacological inhibition of mPTP delays axonal degeneration. A, Transverse sections of nerve explants stained for NF-H (red) and NF-M (green) isoforms. The NF signals decrease considerably after 3 d (3d) in vehicle solution (Veh) and are almost completely lost by 6 d (6d). The CsA (20 μm)-treated explants show preservation of axonal proteins over 3 and 6 d in culture. Scale bar, 20 μm. B, Quantification of NF-H-positive axons in explant cross sections as shown in A, expressed as axons per 100 μm2. Statistically significant protection is seen after CsA treatment at both 3 and 6 d (n = 3 per each group; #p < 0.05 by Student's t test compared with 0 d; *p < 0.05 by Student's t test compared with 3 d vehicle; error bars indicate SEM). C, Nerve explants after different incubation times and conditions were analyzed by Western blot. Treatment with CsA delays the decrease of NF-H at 3 and 6 d compared with untreated nerves. The same effect is seen for neurofilament light (NF-L) at 3 d, but less overall protection was seen for NF-L in the 6 d incubation samples. Histone H3 (His-H3) was used as a loading control. D, Densitometry of NF-H normalized to His-H3 and expressed as percentage of NF-H at day 0. Significant protection at 20 μm CsA for neurofilament decay is seen at 3 d and also at 6 d (n = 3 per each group; #p < 0.05 by Student's t test compared with 0 d; *p < 0.05 by Student's t test compared with 3 d vehicle; error bars indicate SEM). E, Transverse sections of nerve explants stained for NF-H (red) and NF-M (green). Incubation with the mPTP blockers DIDS (250 μm), R.Red (50 μm), and BAPTA-AM (100 μm) for 3 d delays axonal degeneration. Scale bar, 20 μm. F, Quantification of axons positive for NF-H in explant cross sections, expressed as axons per 100 μm2 (n = 3 per group; #p < 0.05 by Student's t test compared with 0 d; *p < 0.05 by Student's t test compared with 3 d vehicle; error bars indicate SEM). G, By Western blot analyses for NF-H, each compound appeared to delay the decay of intact NF-H in the nerve explants over 3 d incubation. H, Densitometry of NF-H normalized to His-H3 and expressed as a percentage of NF-H at day 0. Significant protection of NF decay is seen at 3 d (n = 3 per group; #p < 0.05 by Student's t test compared with 0 d; *p < 0.05 by Student's t test compared with 3 d vehicle; error bars indicate SEM).
Figure S1.
Figure S1.
Wallerian degeneration ex vivo. Sciatic nerves from WT (wild-type) and Wlds mice were placed in culture dishes and axonal degeneration assessed using quantitative immunofluorescence and Western blot analyses. A, B, WT (A) and Wlds (B) sciatic nerve cross sections immunostained for neurofilament isoforms; red, NF-H heavy chain (top row); green, NF-M medium chain (middle row); merged signals (bottom row). The incubation times for each column of images are indicated in the bottom row. A′, B′, Quantification of axon density and area are shown correspondingly in the graph [black symbols and ordinates, axonal density (number of axons per endoneurium area); red symbols and ordinates, endoneurial area occupied by axons (aggregate axonal area per area of nerve)]. A–B′, The population of WT neurofilament positive axons falls dramatically within the first 3 d in culture (A, A′), while Wlds nerves show clear preservation of axons (B, B′), which is a phenotypic feature of this strain. Scale bar, 20 µm. C, D, WT (C) and Wlds (D) Western blots of neurofilament heavy and light chain expression (NF-H and NF-L, respectively). NF chains fall to very low levels within 3 d in WT nerves (C), whereas in Wlds nerves, NF chains remain high for over a week (D). E, Quantification of NF-H band. NF-H falls to traces in 3 d in WT nerves (red curve), while in Wlds nerves (black curve) it remains high for over a week. In a given strain, immunofluorescence or Western blot yields a similar time course of degeneration.
See this image and copyright information in PMC

Erratum for

Publication types

LinkOut - more resources