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. 2021 Jun;71(3):396-403.
doi: 10.1270/jsbbs.20153. Epub 2021 Jun 19.

Identification and validation of quantitative trait loci associated with seed yield in soybean

Naoya Yamaguchi  1 , Fumio Taguchi-Shiobara  2 , Yumi Sato  3 , Mineo Senda  3 , Masao Ishimoto  2 , Fumiko Kousaka  4
Affiliations

Identification and validation of quantitative trait loci associated with seed yield in soybean

Naoya Yamaguchi et al. Breed Sci. 2021 Jun.

Abstract

In soybean [Glycine max (L.) Merrill], the genetic analysis of seed yield is important to aid in the breeding of high-yielding cultivars. Seed yield is a complex trait, and the number of quantitative trait loci (QTL) involved in seed yield is high. The aims of this study were to identify QTL associated with seed yield and validate their effects on seed yield using near-isogenic lines. The QTL analysis was conducted using a recombinant inbred line population derived from a cross between Japanese cultivars 'Toyoharuka' and 'Toyomusume', and eight seed yield-associated QTL were identified. There were significant positive correlations between seed yield and the number of favorable alleles at QTL associated with seed yield in the recombinant inbred lines for three years. The effects of qSY8-1, a QTL promoting greater seed yield, was validated in the Toyoharuka background. In a two-year yield trial, the 100-seed weight and seed yield of Toyoharuka-NIL, the near-isogenic line having the Toyomusume allele at qSY8-1, were significantly greater than those of Toyoharuka (106% and 107%, respectively) without any change for days to flowering and maturity. Our results suggest that qSY8-1 was not associated with maturity genes, and contributed to the 100-seed weight.

Keywords: 100-seed weight; quantitative trait locus; seed yield; soybean.

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Figures

Fig. 1.
Fig. 1.
Frequency distribution of seed yield in each year. (A) 2009. (B) 2011. (C) 2012. Black and white arrows indicate the seed yields of Toyomusume and Toyoharuka, respectively. The seed yields of the parental cultivars are shown in parentheses (average ± standard deviation).
Fig. 2.
Fig. 2.
Box plot of seed yield in the recombinant inbred lines for each trial year. Crosses (×) indicate the average values. (A) 2009. (B) 2011. (C) 2012. Pearson’s correlation coefficient was determined between seed yield and the number of favorable alleles at each QTL associated with seed yield in each year. *** Significant at P < 0.001.
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