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. 2022 Jul;135(4):565-577.
doi: 10.1007/s10265-021-01357-6. Epub 2021 Nov 15.

The difficulty of estimating the electron transport rate at photosystem I

Riu Furutani  1   2 , Miho Ohnishi  1   2 , Yuki Mori  1 , Shinya Wada  1   2 , Chikahiro Miyake  3   4
Affiliations

The difficulty of estimating the electron transport rate at photosystem I

Riu Furutani et al. J Plant Res. 2022 Jul.

Abstract

It is still a controversial issue how the electron transport reaction is carried out around photosystem I (PSI) in the photosynthetic electron transport chain. The measurable component in PSI is the oxidized P700, the reaction center chlorophyll in PSI, as the absorbance changes at 820-830 nm. Previously, the quantum yield at PSI [Y(I)] has been estimated as the existence probability of the photo-oxidizable P700 by applying the saturated-pulse illumination (SP; 10,000-20,000 µmol photons m-2 s-1). The electron transport rate (ETR) at PSI has been estimated from the Y(I) value, which was larger than the reaction rate at PSII, evaluated as the quantum yield of PSII, especially under stress-conditions such as CO2-limited and high light intensity conditions. Therefore, it has been considered that the extra electron flow at PSI was enhanced at the stress condition and played an important role in dealing with the excessive light energy. However, some pieces of evidence were reported that the excessive electron flow at PSI would be ignorable from other aspects. In the present research, we confirmed that the Y(I) value estimated by the SP method could be easily misestimated by the limitation of the electron donation to PSI. Moreover, we estimated the quantitative turnover rate of P700+ by the light-to-dark transition. However, the turnover rate of P700 was much slower than the ETR at PSII. It is still hard to quantitatively estimate the ETR at PSI by the current techniques.

Keywords: P700; Photosynthetic electron transport reaction; Photosystem I; Y(I).

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