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. 2022:2396:1-17.
doi: 10.1007/978-1-0716-1822-6_1.

Methods for the Development of Recombinant Microorganisms for the Production of Natural Products

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Methods for the Development of Recombinant Microorganisms for the Production of Natural Products

Alexander Perl et al. Methods Mol Biol. 2022.

Abstract

Metabolic engineering strives to develop microbial strains that are capable of producing a target chemical in a biological organism. There are still many challenges to overcome in order to achieve titers, yields, and productivities necessary for industrial production. The use of recombinant microorganisms to meet these needs is the next step for metabolic engineers. In this chapter, we aim to provide insight on both the applications of metabolic engineering for natural product biosynthesis as well as optimization methods.

Keywords: Metabolic engineering; Natural products; Recombinant microorganisms.

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References

    1. Stephanopoulos G (2012) Synthetic biology and metabolic engineering. ACS Synth Biol 1(11):514–525. https://doi.org/10.1021/sb300094q - DOI - PubMed
    1. Tyo KE, Alper HS, Stephanopoulos GN (2007) Expanding the metabolic engineering toolbox: more options to engineer cells. Trends Biotechnol 25(3):132–137. https://doi.org/10.1016/j.tibtech.2007.01.003 - DOI - PubMed
    1. Cobb RE, Wang Y, Zhao H (2015) High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system. ACS Synth Biol 4(6):723–728. https://doi.org/10.1021/sb500351f - DOI - PubMed
    1. Schweder T, Krüger E, Xu B, Jürgen B, Blomsten G, Enfors S-O, Hecker M (1999) Monitoring of genes that respond to process-related stress in large-scale bioprocesses. Biotechnol Bioeng 65(2):151–159. https://doi.org/10.1002/(SICI)1097-0290(19991020)65:2<151::AID-BIT4&g... - DOI - PubMed
    1. George S, Larsson G, Olsson K, Enfors SO (1998) Comparison of the Baker's yeast process performance in laboratory and production scale. Bioprocess Eng 18(2):135–142. https://doi.org/10.1007/PL00008979 - DOI

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