Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2396:175-186.
doi: 10.1007/978-1-0716-1822-6_13.

Non-targeted Lipidomics Using a Robust and Reproducible Lipid Separation Using UPLC with Charged Surface Hybrid Technology and High-Resolution Mass Spectrometry

Giorgis Isaac  1 , Vladimir Shulaev  2 , Robert S Plumb  3
Affiliations

Non-targeted Lipidomics Using a Robust and Reproducible Lipid Separation Using UPLC with Charged Surface Hybrid Technology and High-Resolution Mass Spectrometry

Giorgis Isaac et al. Methods Mol Biol. 2022.

Abstract

Lipids play an important role in the energy storage, cellular signaling, and pathophysiology of diseases such as cancer, neurodegenerative diseases, infections, and diabetes. Due to high importance of diverse lipid classes in human health and disease, manipulating lipid abundance and composition is an important target for metabolic engineering. The extreme structural diversity of lipids in real biological samples is challenging for analytical techniques due to large difference in physicochemical properties of individual lipid species. This chapter describes lipidomic analysis of large sample sets requiring reliable and robust methodology. Rapid and robust methods facilitate the support of longitudinal studies allowing the transfer of methodology between laboratories. We describe a high-throughput reversed-phase LC-MS methodology using Ultra Performance Liquid Chromatography (UPLC®) with charged surface hybrid technology and accurate mass detection for high-throughput non-targeted lipidomics. The methodology showed excellent specificity, robustness, and reproducibility for over 100 LC-MS injections.

Keywords: LC-MS; Non-targeted lipidomics.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Reynolds KB, Taylor MC, Zhou XR, Vanhercke T, Wood CC, Blanchard CL, Singh SP, Petrie JR (2015) Metabolic engineering of medium-chain fatty acid biosynthesis in Nicotiana benthamiana plant leaf lipids. Front Plant Sci 6:164. https://doi.org/10.3389/fpls.2015.00164 - DOI - PubMed - PMC
    1. Park YK, Nicaud JM (2020) Metabolic engineering for unusual lipid production in Yarrowia lipolytica. Microorganisms 8(12):1937. https://doi.org/10.3390/microorganisms8121937 - DOI - PMC
    1. Correa SM, Alseekh S, Atehortua L, Brotman Y, Rios-Estepa R, Fernie AR, Nikoloski Z (2020) Model-assisted identification of metabolic engineering strategies for Jatropha curcas lipid pathways. Plant J 104(1):76–95. https://doi.org/10.1111/tpj.14906 - DOI - PubMed
    1. Wang J, Ledesma-Amaro R, Wei Y, Ji B, Ji XJ (2020) Metabolic engineering for increased lipid accumulation in Yarrowia lipolytica - a review. Bioresour Technol 313:123707. https://doi.org/10.1016/j.biortech.2020.123707 - DOI - PubMed
    1. Pouvreau B, Blundell C, Vohra H, Zwart AB, Arndell T, Singh S, Vanhercke T (2020) A versatile high throughput screening platform for plant metabolic engineering highlights the major role of ABI3 in lipid metabolism regulation. Front Plant Sci 11:288. https://doi.org/10.3389/fpls.2020.00288 - DOI - PubMed - PMC

LinkOut - more resources