doi: 10.1073/pnas.84.20.7000.
Cloning and expression of T4 DNA polymerase
Affiliations
- PMID: 3478676
- PMCID: PMC299216
- DOI: 10.1073/pnas.84.20.7000
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Cloning and expression of T4 DNA polymerase
Proc Natl Acad Sci U S A.
1987 Oct.
Abstract
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.
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