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. 1987 Oct;84(20):7000-4.
doi: 10.1073/pnas.84.20.7000.

Cloning and expression of T4 DNA polymerase

T C Lin  1 , J Rush, E K Spicer, W H Konigsberg
Affiliations

Cloning and expression of T4 DNA polymerase

T C Lin et al. Proc Natl Acad Sci U S A. 1987 Oct.

Abstract

The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

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References

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