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. 2021 Dec;12(2):12323-12331.
doi: 10.1080/21655979.2021.2000257.

Experimental study of fat derived pellets promoting wound healing in rats

Li Zeng  1 , Shaolong Zhou  2 , Chen Chen  2 , Lin-Hua Zhou  2 , Xiujiang Shi  2 , Zongjian Wu  2 , Sheng-Kang Luo  1
Affiliations

Experimental study of fat derived pellets promoting wound healing in rats

Li Zeng et al. Bioengineered. 2021 Dec.

Abstract

To observe the effect of fat-derived pellets (FDP) on wound healing in rats, the inguinal fat of rats was obtained, and the FDP were obtained after centrifugation. The cell activity and growth factor secretion of FDP were measured. The wounds in rats were created, and FDP was used to treat the wounds of rats. The phenotype of macrophages and the expression of angiogenic factors expression in wounds were measured. The cell viability in FDP remains in high level after centrifugation and the expression of vascular endothelial growth factor (VEGF) and Basic Fibroblast Growth Factor (bFGF) from FDP was observed in vitro. The FDP significantly promoted the wound healing of rats compared with that in control groups. Moreover, the expression of M2 macrophages and VEGF in FDP group were significantly higher than that in the control group. FDP is a kind of stem cell product, which can be obtained from adipose tissue by physical centrifugation. The cytotherapeutic effect of FDP makes it a promising product for wound healing in clinics.

Keywords: Stem cell; growth factors; macrophages; physical method; wound healing.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Fat Tissue Derived Pellets (FDP)
Figure 2.
Figure 2.
(a): Cell activity in FDP. (b): Results of FDP secreting VEGF and bFGF in vitro
Figure 3.
Figure 3.
(a): Alizarin red staining for osteogenic differentiated cells. (b): Toluidine blue staining for chondroblast differentiation cells. (c): Oil red O staining for adipogenic differentiated cells
Figure 4.
Figure 4.
(a):General picture of wound surface of rats (b):Ratio of residual wound area to original wound area. *P < 0.05. **P < 0.01
Figure 5.
Figure 5.
(a): On day 1, day 14, day 21, M1M2 macrophages were stained. (b): Quantitative results of M2/M1 ratio on day 21. (c): VEGF gene expression results. *P < 0.05.***P < 0.001.Scale = 200um
Figure 6.
Figure 6.
(a) Expression levels of FGF-1 in FDP group and control group. (b) Relative expression level of collagenase 1 in wound skin tissue. *P < 0.05. **P < 0.01
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