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. 2021 Nov;172(1):96-99.
doi: 10.1007/s10517-021-05339-5. Epub 2021 Nov 18.

Morphofunctional Properties of Corneal Stromal Cells

M A Surovtseva  1 , O V Poveshchenko  2 , K Yu Krasner  3 , I I Kim  2 , A P Lykov  2 , N A Bondarenko  2 , L A Shul'mina  2 , A N Trunov  3 , V V Chernykh  3
Affiliations

Morphofunctional Properties of Corneal Stromal Cells

M A Surovtseva et al. Bull Exp Biol Med. 2021 Nov.

Abstract

Human corneal stromal cells were isolated by enzymatic digestion from a new source, lenticules obtained during laser vision correction by the ReLEx SMILe method. The resulting culture was mainly presented by fibroblast-like cells with a phenotype CD90-/CD73+/CD105+/keratocan-/lumican-/ALDH1A1+ that differentiate into keratocytes in a specialized medium. The concentration of fetal calf serum-derived growth factors affects the rate of proliferation, production of erythropoietin and brain neurotrophic factor by corneal fibroblasts, and to a lesser extent, their migration activity and production of extracellular matrix components. Thus, the high functional potential of fibroblast-like cells isolated from stromal lenticles can be used to develop cell technologies in ophthalmology.

Keywords: corneal fibroblasts; keratocytes; migration; proliferation.

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