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. 2022 Aug;19(5):1243-1252.
doi: 10.1111/iwj.13719. Epub 2021 Nov 17.

The healing dynamics of non-healing wounds using cryo-preserved amniotic membrane

Alzbeta Svobodova  1 , Vojtech Horvath  2 , Ingrida Smeringaiova  3 , Joao Victor Cabral  3 , Martina Zemlickova  4 , Radovan Fiala  5 , Jan Burkert  5   6 , Denisa Nemetova  6 , Petr Stadler  2 , Jaroslav Lindner  1 , Jan Bednar  3 , Katerina Jirsova  3   6
Affiliations

The healing dynamics of non-healing wounds using cryo-preserved amniotic membrane

Alzbeta Svobodova et al. Int Wound J. 2022 Aug.

Abstract

We evaluated the effect of the application of cryo-preserved amniotic membrane on the healing of 26 non-healing wounds (18 patients) with varying aetiologies and baseline sizes (average of 15.4 cm2 ), which had resisted the standard of care treatment for 6 to 456 weeks (average 88.8 weeks). Based on their average general responses to the application of cryo-preserved AM, we could differentiate three wound groups. The first healed group was characterised by complete healing (100% wound closure, maximum treatment period 38 weeks) and represented 62% of treated wounds. The wound area reduction of at least 50% was reached for all wounds in this group within the first 10 weeks of treatment. Exactly 19% of the studied wounds responded partially to the treatment (partially healed group), reaching less than 25% of closure in the first 10 weeks and 90% at maximum for extended treatment period (up to 78 weeks). The remaining 19% of treated wounds did not show any reaction to the AM application (unhealed defects). The three groups have different profiles of wound area reduction, which can be used as a guideline in predicting the healing prognosis of non-healing wounds treated with a cryo-preserved amniotic membrane.

Keywords: cryo-preserved amniotic membrane; healing dynamics; non-healing wounds.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Examples of wound closing. A, healed (H) wound (D15, venous leg ulcer); B, partially healed (PH) wound (D19, venous leg ulcer); C, wound with no reaction (D25, defect after fasciotomy). W0: the wound state after 24, 456, and 100 weeks of outpatient care with SOC treatment for A, B, and C, respectively. W: number of weeks of treatment with AM
FIGURE 2
FIGURE 2
Wound closure evolution. WAR progress for healed A, partly healed B, and unhealed C, defects. D, comparison of the averages of the three groups together with fitted asymptotic functions and their parameters for H and PH and correlation coefficients (R2). For A, B, and C, the closed and open markers reflect visits with or without AM application, respectively
FIGURE 3
FIGURE 3
Pain level evolution during the AM treatment. Average value ± SD from all patients on a scale from 0 (no pain) to 10 (the worst pain) at week (W) 0, 1, 5, and 10 of treatment
See this image and copyright information in PMC

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