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. 2022 Apr;194(4):1527-1545.
doi: 10.1007/s12010-021-03696-5. Epub 2021 Nov 18.

Evaluation of Substitution of Small Branches with Roots of Desmodium gangeticum (Physicochemical Analysis, HPLC, and GC-MS Profiling) and In Silico Study of Pterocarpans for Pharmacological Target

Ajay Kumar Meena  1 , Mohit Motiwale  2 , R Ilavarasan  3 , Ayyam Perumal  3 , Ravindra Singh  4 , N Srikanth  4 , K S Dhiman  4
Affiliations

Evaluation of Substitution of Small Branches with Roots of Desmodium gangeticum (Physicochemical Analysis, HPLC, and GC-MS Profiling) and In Silico Study of Pterocarpans for Pharmacological Target

Ajay Kumar Meena et al. Appl Biochem Biotechnol. 2022 Apr.

Abstract

The present research article proposes a conservative approach for the Desmodium gangeticum by using small branches instead of roots because the plant has many important chemical constituents that show different medicinal activity, so the plant's consumption is high. We studied here comparative preliminary phytochemical screening test and physicochemical analysis. The successive soxhlet extraction method was used for the successive extraction of roots and small branches with different solvents for comparative chemical profile study by HPLC and GC-MS. It was observed that many peaks in roots and small branches of the plant sample were almost similar, and the retention time of each peak in roots coincided with the retention of small branches of the sample. Therefore, the similarity was observed in roots and small branches of the Desmodium gangeticum plant in HPLC and GC-MS. The results obtained from HPLC analysis show that roots contain 0.00116% and small branches have 0.00026% of caffeic acid in Desmodium gangeticum. The small branches may have almost similar active chemical constituents like roots. In silico molecular docking study revealed that this plant's principal chemical constituents, pterocarpans, could be inhibitors of protein tyrosine phosphate kinase.

Keywords: Desmodium gangeticum; GC–MS; Molecular docking; PTPN1; Plant part substitution; Pterocarpans.

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References

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