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. 2022 Mar 24;59(3):2102102.
doi: 10.1183/13993003.02102-2021. Print 2022 Mar.

In vivo microevolution of Mycobacterium tuberculosis and transient emergence of atpE _Ala63Pro mutation during treatment in a pre-XDR TB patient

Arash Ghodousi  1   2 , Alamdar Hussain Rizvi  3 , Faisal Masood Khanzada  3 , Nasim Akhtar  4   5 , Abdul Ghafoor  4 , Alberto Trovato  2 , Daniela Maria Cirillo  1   2 , Sabira Tahseen  6
Affiliations

In vivo microevolution of Mycobacterium tuberculosis and transient emergence of atpE _Ala63Pro mutation during treatment in a pre-XDR TB patient

Arash Ghodousi et al. Eur Respir J. .

Abstract

This letter describes microevolution of a pre-XDR MTB strain isolated from a pulmonary TB patient over an 18-month exposure to BDQ. MDR-TB therapies with BDQ require a functional background regimen to prevent emergence of additional resistance. https://bit.ly/3D05qT9

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Conflict of interest statement

Conflict of interest: None declared.

Figures

FIGURE 1
FIGURE 1
a) Shown is a summary of genotypic drug resistance (based on whole genome sequencing (WGS)). b) Bedaquiline minimum inhibitory concentrations (MICs) performed in 7H11 and MGIT, and treatment monitoring during the bedaquiline-containing regimen. c) Growth curves of standing liquid cultures of Mycobacterium tuberculosis strains including H37Rv, baseline isolates (isolate A) and mutant strains (isolates B and E). The experiment was repeated three times giving comparable results and the mean values were plotted. Bdq: bedaquiline; Mfx: moxifloxacin; Lzd: linezolid; Cfz: clofazimine; Cs: cycloserine: Cm: capreomycin; Dlm: delamanid; Eto: ethionamide; Z: pyrazinamide; XDR: extensively drug resistant.
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References

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    1. World Health Organization . Technical Report on Critical Concentrations for Drug Susceptibility Testing of Medicines used in the Treatment of Drug-resistant Tuberculosis. Geneva, World Health Organization, 2018.
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