. 2021;12(5):48-54.

doi: 10.17691/stm2020.12.5.05. Epub 2020 Oct 28.

Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

A G Velichanskaya¹, D A Abrosimov², M L Bugrova³, A V Kazakov⁴, E V Pogadaeva⁵, A M Radaev¹, N V Blagova⁶, T I Vasyagina⁷, I L Ermolin⁸

Affiliations

¹ Associate Professor, Department of Histology, Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
² Senior Lecturer, Department of Histology, Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
³ Associate Professor, Head of the Department of Electron Microscopy, Central Research Laboratory; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁴ Researcher, Research Laboratory, Clinic of Cardiology, Angiology, and Intensive Care; Saarland University, Saarbrücken Campus, Saarbrücken, 66123, Germany.
⁵ Senior Laboratory Assistant, Department of Histology, Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁶ Senior Lecturer, Department of Histology Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁷ Senior Researcher, Department of Electron Microscopy, Central Research Laboratory; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁸ Professor, Head of the Department of Histology, Cytology, and Embryology Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.

PMID: 34796004
PMCID: PMC8596261
DOI: 10.17691/stm2020.12.5.05

Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

A G Velichanskaya et al. Sovrem Tekhnologii Med. 2021.

. 2021;12(5):48-54.

doi: 10.17691/stm2020.12.5.05. Epub 2020 Oct 28.

Authors

A G Velichanskaya¹, D A Abrosimov², M L Bugrova³, A V Kazakov⁴, E V Pogadaeva⁵, A M Radaev¹, N V Blagova⁶, T I Vasyagina⁷, I L Ermolin⁸

Affiliations

¹ Associate Professor, Department of Histology, Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
² Senior Lecturer, Department of Histology, Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
³ Associate Professor, Head of the Department of Electron Microscopy, Central Research Laboratory; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁴ Researcher, Research Laboratory, Clinic of Cardiology, Angiology, and Intensive Care; Saarland University, Saarbrücken Campus, Saarbrücken, 66123, Germany.
⁵ Senior Laboratory Assistant, Department of Histology, Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁶ Senior Lecturer, Department of Histology Cytology, and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁷ Senior Researcher, Department of Electron Microscopy, Central Research Laboratory; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁸ Professor, Head of the Department of Histology, Cytology, and Embryology Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.

PMID: 34796004
PMCID: PMC8596261
DOI: 10.17691/stm2020.12.5.05

Abstract

The aim of the study was to compare two types of conduits made of either non-resorbable Reperen or resorbable Tissucol for their effects on the regeneration of the rat sciatic nerve under conditions of stump diastasis.

Materials and methods: The experiments were carried out using outbred white male rats of the reproductive age (n=14). The animals were divided into three groups: group 1, intact (n=5), used for studying the morphology of the sciatic nerve; group 2 (n=4) - nerve plastic surgery was performed using a conduit made of non-resorbable Reperen; group 3 (n=5) - surgery was performed using a conduit made of resorbable Tissucol. The animals were anesthetized with isoflurane. After a complete transection of the sciatic nerve in the middle third of the thigh, its stumps were inserted into a conduit of an internal diameter of 2 mm and a length of 10 mm, filled with saline. Diastasis of 5 mm in length was created by spreading the nerve ends and securing the epineurium to the tube edges with 8/0 polypropylene sutures. A total count of myelinated nerve fibers was performed in the area of repair (tubulation) and the distal part of the nerve; the formation of connective tissue sheaths was assessed 14 weeks after the operation.

Results: According to the morphological assessment, both types of conduits (resorbable and non-resorbable) caused the similar number of fibers to restore in the distal part of the repaired nerve; clinical characteristics of the animals in both groups were close to each other and to the norm.

Conclusion: The results allow us to consider the conduit made of non-resorbable Reperen as a device promising for neuroplasty along with the resorbable conduit made of Tissucol.

Keywords: Reperen; Tissucol Kit; conduit for nerve repair; nerve regeneration; peripheral nerve.

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Conflict of interest statement

Conflict of interest. The authors declare that they have no conflicts of interest to report.

Figures

**Figure 1. Cross-section of the rat sciatic nerve:**
(a) in an intact animal; (b) regenerated in a conduit from Reperen after 14 weeks; (c) regenerated in a conduit from Tissucol after 14 weeks; A — nerve fibers, B — perineurium, C — epineurium; staining with methylene blue and fuchsine

**Figure 2. The rat sciatic nerve upon 14 weeks of regeneration:**
(a) in a Reperen-based conduit; (b) in a Tissucol-based conduit (marked with a staple)

**Figure 3. Regenerated rat sciatic nerve 1 mm distal to the conduit (after 14 weeks):**
(a) with Reperen; (b) with Tissucol; A — nerve fibers, C — epineurium; staining with methylene blue and fuchsine

**Figure 4. Position of the right hind limb after 14 weeks of nerve regeneration:**
(a) with a conduit made of Reperen; (b) with a conduit made of Tissucol

See this image and copyright information in PMC

Cited by

Sciatic Nerve Plastic Surgery using Autologous Adipose Tissue.
Velichanskaya AG, Bugrova МL, Pogadaeva EV, Ermolina EA, Yudintsev AV, Ermolin IL. Velichanskaya AG, et al. Sovrem Tekhnologii Med. 2023;15(1):30-36. doi: 10.17691/stm2023.15.1.04. Epub 2023 Jan 28. Sovrem Tekhnologii Med. 2023. PMID: 37388749 Free PMC article.
Comparison of three different strategies to treat sciatic nerve regeneration: an experimental study.
Smaniotto PH, Camargo CP, Kubrusly MS, Gemperli R. Smaniotto PH, et al. Acta Cir Bras. 2022 Aug 12;37(5):e370501. doi: 10.1590/acb370501. eCollection 2022. Acta Cir Bras. 2022. PMID: 35976339 Free PMC article.

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Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

Affiliations

Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

Authors

Affiliations

Abstract

Conflict of interest statement

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